LOCUS       BG066558                 566 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3045D01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3045D01 3', mRNA sequence.
ACCESSION   BG066558
VERSION     BG066558.1
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished
COMMENT     Other_ESTs: H3045D01-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3045  row: D  column: 01
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3045D01-3"
                     /db_xref="taxon:10090"
                     /clone="H3045D01"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          158 a          114 c          133 g          161 t
ORIGIN      
        1 ctgcaataga tttgggtctc taagaaatga ctacattgaa ttcctatccc taatactgtt
       61 agagatttgg gagctgatta gttcacgtag tgctgttcct ggttgtcaac ttgactatat
      121 ctggaatgaa ctacaatcca gaattggaag gctcacctgt gatccagatc ttgaggctgg
      181 gagaaataag tttctgacat ggattttggc ctggagattt tgaggcatag tggctatgag
      241 tcccaggaat ccaaggcaag gagagctgag tttaaggtca tctgggaaaa agcaagtcag
      301 atccaggcat ggcagcacac acctttaatc tgatccacac cttctgctgg agaactacag
      361 aaggacattg gaagaaggaa gactctctct tctttacctg cttgctttgt gggactgagc
      421 aagtgctaga tccttggact tcattcacag ctgctgctga tcattgttag ggagttggac
      481 tgcagtcatc aacaaattcc tttactatag agagaatgcc catatgttct gtgactctag
      541 agaaccctga ctaacacatt tcataa
//