LOCUS BG066554 707 bp mRNA linear EST 12-MAY-2010 DEFINITION H3045C09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3045C09 3', mRNA sequence. ACCESSION BG066554 VERSION BG066554.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 707) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066554.1. Other_ESTs: H3045C09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3045 row: C column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..707 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3045C09-3" /db_xref="taxon:10090" /clone="H3045C09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 171 a 170 c 158 g 208 t ORIGIN 1 ctctaatcat catttaagaa tgggatttta gccaagttct tacaagcaga aaacacagtt 61 ctgtcaggaa gttaatgttc tcgatatggc agagcagccc cagacaataa caaccaggtt 121 aaaggctaag aagagctgca atctccttat cattgggcta ggaaatggct taagggagtc 181 accgttcaga aaacgataaa atataagccc agacttaaac atcttcagct gtgaagtcag 241 tatgtggtgc tttgatttct tcctgcatca tgcaatttct aagttccatt aaaaacatta 301 tcttcggttt cttcttgggc aactactgct catgaaaggg tcatcgtcat catcctcatc 361 tgattcaaag tctacccctt tggctgtctg gctctgggac atccgtttgc tagatgttgt 421 gcccgaccac ctttgatcag ccctggaatt ggtaggaaaa atgtcatctt cgtcagagtc 481 atccacctca atggtctctg agtaattctt aggggctgca tttctagaag gctgttgtcg 541 ggtagatctg aaagcgtcta taatggacat gtttctagat gttgatgagg tggcctttga 601 gctcctgcct cgagtagtga tctccagccc agtgtctcgg cctctctgag agcctcctct 661 aggtgccgag ctctgccctc tggctcctct tcgccctcgg cctcggc //