LOCUS BG066543 701 bp mRNA linear EST 12-MAY-2010 DEFINITION H3045B07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3045B07 3', mRNA sequence. ACCESSION BG066543 VERSION BG066543.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 701) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066543.1. Other_ESTs: H3045B07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3045 row: B column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..701 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3045B07-3" /db_xref="taxon:10090" /clone="H3045B07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 195 a 179 c 182 g 145 t ORIGIN 1 aaaaaggcaa aggaaaacaa accattcttg aagtgtggtg ggcaggtaag agtggaggtg 61 cttgggagag tccttaccac tttcagggct gtgacctttg tcctggattc ctccagggac 121 tgggtgaagc agaggggtag cagtccacat agacccaaaa gcctgagaca caagtttcct 181 cttgggcctg gaacccacat ccttgtgtag aatgaggctc tggaaccacc ctcctccagc 241 tcacaggaca caggctggaa gctccattgc tggggccaaa gctatgggtc atccttgacc 301 ttggtaacag aggtgcaggc cccacgatga tcagtttcca tttgggccct gctaactcaa 361 ccgccaaagc tcaggaaagt aagccacagc cttagcaact gaggcgcctt cctatcctct 421 acggttggga cctgcctgcc aggcttacct gcccaggtag ccgggacagt atacatagtc 481 ccaaatacac agagtcccaa ggttccaaga ccatgttacc caagttaaat ctctttaata 541 tcccaataca aagtcttgat gcaaaaagac aatgagaaaa ccctgggaag ttgagaggag 601 gggttttata aataaaccca ccgagcagct tttcaagggg aaagggggga cgcaaaggag 661 ccaaggaagc gtcagtccac agctcctagg atttgccctt g //