LOCUS BG066539 729 bp mRNA linear EST 12-MAY-2010
DEFINITION H3045B03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3045B03 3', mRNA sequence.
ACCESSION BG066539
VERSION BG066539.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 729)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG066539.1.
Other_ESTs: H3045B03-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3045 row: B column: 03
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..729
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3045B03-3"
/db_xref="taxon:10090"
/clone="H3045B03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 167 a 143 c 231 g 188 t
ORIGIN
1 gggccagaaa caaactttta ttgcaaaacg ggaaccttca agtcacaaag aggtcagcta
61 tggcttctgt cctgataagg agggctggaa acagtaacag cactcccctg catcatccct
121 gtgaggccag ttagtccttc caggaggagg atacatttcc agcactctca tgaggacttg
181 caggcagctc agagatcaga atgcgtttga gagcaggccc ctgataacat gactgggaaa
241 ccctttttct ttgggtcgct ggtcctcatt tgtagaaggt ctgtgtgcac aatcacagac
301 caggaaacct ttatatttgg gaacatttgt tggaaggagc gtgctggagg ggcatgggtg
361 tggacgtgac tggggaatag tatgtcgtca cggtgtgcct cagtgggaag aagtttggaa
421 gggccaagat cttgtcacat acggtttggc tgtgtggatt actggctggt ggccgttgat
481 agagccactc atgagcctgg atggtggtga ggtcatccat gggagtagac agaacatcgt
541 tctggctgtg acatgacccc agctggggct tgctgttttc tatggcgggg atccagcaac
601 agcggtgctg agcgtgttag acagagttaa gagtcctgaa tacggttggg ttgtttgtgt
661 gtttgtgtag gcagtcgctg gattagagtt gtgagcatac agagtcgtgt ggtggacagc
721 ggggtatct
//