LOCUS BG066489 530 bp mRNA linear EST 12-MAY-2010 DEFINITION H3043E11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3043E11 3', mRNA sequence. ACCESSION BG066489 VERSION BG066489.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 530) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066489.1. Other_ESTs: H3043E11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3043 row: E column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..530 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3043E11-3" /db_xref="taxon:10090" /clone="H3043E11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 129 a 133 c 111 g 157 t ORIGIN 1 ctttgggctc cttattctct gggtccccag gaacaatctc cattggtctc tgttctcctg 61 agttgttttc tgcggcatcc acaatgttgt cttcatccgc ttgttcagca atctctttct 121 cctcaagctc accgttactt tcttcaactg gagtgctgtc tttaggctct gaggacagca 181 tctcagcaga aaatgtccca ttgaggagac tgtttacttt gttgagaggg aactcgtaaa 241 gaccactgag gaaagatttg ggaaatccaa aacatgctgt tgccgcccga acgggtccat 301 ctccaggata cagctgaata atggagccaa agcctcccat tcgttccatc atggcaccta 361 gcaccaagcc cgagcacgtt tccatgacaa tcattttatt gccggcacgg atatttccca 421 acgtcaacat ctgggctagt gtatcatatc tcatgtggtt aatttttcca ggttctcttg 481 cataatacat aattgaaaga atacgggtag atggcttcaa gatagtaacg //