LOCUS BG066409 545 bp mRNA linear EST 12-MAY-2010 DEFINITION H3042F10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3042F10 3', mRNA sequence. ACCESSION BG066409 VERSION BG066409.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 545) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066409.1. Other_ESTs: H3042F10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3042 row: F column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..545 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3042F10-3" /db_xref="taxon:10090" /clone="H3042F10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 129 a 123 c 166 g 127 t ORIGIN 1 gattcaaaca atttactgtg acaattgcca aggctgaggt caagggtggg tagactgtgg 61 tcaaggacgg cttcccaacc atagacagag cttcatcgcc tggaagccac tgaacacagc 121 agagacctag taccatgggc tagaaggaga ggtcggtctc acgcttggtc cctgaaatgc 181 catctcctcc actctagaag gttctgagtg atgaatagca tggggggggg ggaagaaacc 241 cttgccacaa atggctcctt gcagcctgcc ctaaagtgga cctctcctga ttagattctg 301 tatctagcag agattggctc agtcagagag taggtgttgc tcataactgg cttgaactcc 361 ctttccagag tgagtactgc caaggctgag gtcaagggtg gatagactgt ggtcaaggac 421 ggattccgag gtggctgggg tgctgggctt taggcataca tcgtcagtgt gggtccattg 481 ctcctcatgg cttcgggcag atcaggggct ctgaggaaac tgctggaact ccgggtgtgg 541 attaa //