LOCUS BG066372 587 bp mRNA linear EST 12-MAY-2010 DEFINITION H3042B12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3042B12 3', mRNA sequence. ACCESSION BG066372 VERSION BG066372.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 587) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066372.1. Other_ESTs: H3042B12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3042 row: B column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..587 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3042B12-3" /db_xref="taxon:10090" /clone="H3042B12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 121 a 169 c 139 g 158 t ORIGIN 1 ccacactctc gctctctctt ctctccatga ttattgactg ccctgggagc ctgatcacaa 61 accctgcttg gccaaggaag cagacacttc atcggctagt gtggcaagct gtggtgagtc 121 caccatgtcg atgctgcccg tggaagacac attgctgagg tgccgtggag atgtgtcccc 181 agacaccacg ggtgacttat acacaatttc tgctccatgg tctgtcttgg ctttggcatt 241 ctccctgaag gtcagcttgt gggtttcaat cttcttattc cctcctccag ggacgtgggt 301 gatattatcc aaggagccaa tcttcgactg gactctgtcc ttgaagtcca gcttctctga 361 ttttacttcc acctggccac ctcctggctt gtgatggatg ttccctaacg agccacactt 421 ggaggtcact ttgctcaggt ccaccggctt gtagactatt tgcaccagga agcttaggga 481 cctaaacatc ccagaaacac aagacactct catggaaaag caggtgcctg gtccttgtac 541 gtgaccatct ctgtagcaga caactgcctc cacccgggac gtgtttg //