LOCUS BG066204 658 bp mRNA linear EST 12-MAY-2010 DEFINITION H3040B08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3040B08 3', mRNA sequence. ACCESSION BG066204 VERSION BG066204.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 658) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066204.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3040 row: B column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..658 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3040B08-3" /db_xref="taxon:10090" /clone="H3040B08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 142 a 185 c 159 g 172 t ORIGIN 1 ggttttgttt ttgtttgttt gagtagggta tcatgcaacc caggccggct tcaaccccac 61 tatgtagctg tgaatggcct tcaattcctg gtcctctcag ctcagccttc cggggtgttg 121 ggtccacagc tatgtgcctc catacctggc tgctgtttcg ctgctctcag atggagtggc 181 tgagcccttt gtgcctttgg tgggtgtgct tggctggtgt tgcccaccca gacaggtatt 241 ttccagcact tactatgcat aggctactct gggagctaag ccacatggaa agaggccctg 301 gggcgcaagt ggcctttcca agctgtttat tcacaaaata aggccttctc tgaggacacc 361 tactgggtgg cagccagtgt ctcaagtatc aaccctgaac agaaccaaca agacaagccc 421 acactgtggg gtttatacaa gcccctaaca aaggcagcag gcagcaggac atgctcagaa 481 gtacagcata gccaggcagc acctgttgga gcctctcaac accatgtgga tgcagacact 541 ttgtgtatct ccacagcccc accactggcc tttgctgact atcacccctt ccggccaccc 601 acacaaagca tttatctgct tgctgtctct cctgctgcat tttgtataag aatgattc //