LOCUS BG066196 491 bp mRNA linear EST 12-MAY-2010 DEFINITION H3040A09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3040A09 3', mRNA sequence. ACCESSION BG066196 VERSION BG066196.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 491) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066196.1. Other_ESTs: H3040A09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3040 row: A column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..491 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3040A09-3" /db_xref="taxon:10090" /clone="H3040A09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 107 a 128 c 153 g 103 t ORIGIN 1 gccagtgggt acactctttt ctccttgccc actggttcca tgggtgcgct cttccgcatc 61 ggttcctggc tgctgcacca gctcgtgatc ctaaggtctt ctaagagagc ggatgggacg 121 catgtgagac tccagaactg tgcgagtcac ggctgctgct ctgccccagg ctgcacgaat 181 gctaatctct gcatttggcc catgactgca ggaactttcg cagtactcag tctaatgacc 241 ggttctgtag tcgagagggt ggtgcccgaa cccctcgcgg ggaacctcag tggaatcgaa 301 agggagcaac tggaagctcg gagagttggg gcggcagccg ctgtggcctt cgggagtggg 361 gcgctaatgg taagcgagga ctcgacccgg gtgaggacct tagatgaatc caccgaaaag 421 gaccaacaca attgtgccag aggatagagg ggaggataaa aacaccattg aacttggctt 481 ccagtccgga g //