LOCUS BG066164 693 bp mRNA linear EST 12-MAY-2010
DEFINITION H3039F09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3039F09 3', mRNA sequence.
ACCESSION BG066164
VERSION BG066164.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 693)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG066164.1.
Other_ESTs: H3039F09-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3039 row: F column: 09
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..693
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3039F09-3"
/db_xref="taxon:10090"
/clone="H3039F09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 199 a 134 c 161 g 199 t
ORIGIN
1 gcttttatta actttgaatg agatattcat ttgtacatga tcattatctc caagtggcac
61 tttcaaggta tgtaaaagta caccctgcat gacaagtgtc cctccttatt gagctgaata
121 gtgcacacac agggcaatgg agcaggggag ggatgcaggg gcgtgtgaga agtaagaaac
181 tttcttagct ccttgatttt gcttgggggt gaccctgtgg gagagcttta gtaacacatg
241 ttctctgtgg ttctaaaatt acttaatttc atagtcattt tacaataccc atattaagag
301 ttgagataca ataaaagtag aatactttca tctacagtcc cccggaatga aagcactaat
361 gttataaagt atttggcatt gctagatacc gaggttctca tggtagatga gacggagtgg
421 accgagttca cagtgcttac gactcatcag agcagtccat cacttctcgg aaaatatatc
481 tgagtgctct gggtgggtga cttccaagac gggtccaaac gagccctact tccttgtagt
541 aacatttctg tataattctc ctctgtggaa gggttggtca ggacatcaga aggacaatca
601 tagactgtga accacaaaac agtacataag cacaagacac agatctttgc ttccttaggg
661 caattgctgt gaggggatgg tctcatatct gag
//