LOCUS BG065831 737 bp mRNA linear EST 12-MAY-2010 DEFINITION H3035G04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3035G04 3', mRNA sequence. ACCESSION BG065831 VERSION BG065831.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 737) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG065831.1. Other_ESTs: H3035G04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3035 row: G column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..737 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3035G04-3" /db_xref="taxon:10090" /clone="H3035G04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 176 a 197 c 180 g 184 t ORIGIN 1 cagttttaga aaagccttta ttgcaaattc cattctgcat gttgattttt ttttgaagac 61 atcagtgcat cttccctgag cacaaaaatc gagttagacc taagaatagg atctttcttc 121 aatcagcact cttaagtccc tatcatttaa gcaaaatcaa agctagggat gtgatcttgc 181 aggtgaataa aatgctttct tttctcagaa acagcagcag tggttttggt aggatcaaat 241 gtgatctcca ggatcttgct tttggggtgc catgggcagg ctgacatcac agagcttaca 301 aggccagggc ccagtctcac tgagtgtctc agctggctct acttttggcc agagaagggc 361 acagatgcct tgtcgcagcc tgccatgcac gcttcgattt cctccacagt ccggggtaca 421 catgtcagca gctccatgcc gctgtcagtc acgacaacat cttcctcgat ccgcacccca 481 ccgaagttgc ggaacgctgc aggacctctt ggttaaagaa gcaggattgg gccgggtctg 541 ccagggcctg gtccaagagg tggtcaatga agtagatgcc tggctccaca gtcagcacca 601 tgcccggctc caggtgcctt gccgtacgca ggcttcgcag gccaggttca tcgatgcgct 661 ctacaccctc agggtagcct cccacgtcgt gcacatctag gcccagaaag tagccaagtc 721 catgtggcat gaatact //