LOCUS BG065788 842 bp mRNA linear EST 12-MAY-2010
DEFINITION H3035C08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3035C08 3', mRNA sequence.
ACCESSION BG065788
VERSION BG065788.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 842)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG065788.1.
Other_ESTs: H3035C08-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3035 row: C column: 08
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..842
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3035C08-3"
/db_xref="taxon:10090"
/clone="H3035C08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 234 a 218 c 182 g 208 t
ORIGIN
1 caaacactct aaaattggat gatttgtatt taacaacttg ggctccagac agcgtgtcaa
61 acatgaggag tggcagccac atacaccttt cccataaggt ggcagtaatc aaagtacctc
121 actgggtcca gtcctaagcc cctgttggcc cagctacttc ctcttcagta cctccccaac
181 acagacttca atatccaggg ccacctatcc agctgttgtt tttactagtt aggccaacag
241 ggagtggcgg actcctgcat ggatacctcc tgacgggtgc agtggaaggc ctcagtgaca
301 ggctgtgacc accataagga atggaggaga cctctatagc aactgcaact ggccctacat
361 agagcactag aaactattct gatgagggac tttcctgtca ggttccatgc ctttccaacc
421 aacgtgggaa cagcatgaat gggaagacag tgtgtgtcac atgtgtgccc gcccacagga
481 cagaactgca tgcagcattt ccagaatcta ctgcaggacc tttctgcagg ctgcaagaga
541 aagctcagta gcacccatat acctaaccaa aaagcaccag agtatggacc tcaaacatct
601 tgtctgtttt tggcttttcc agacagggtt tctctgtgta gctctggctg tcctggaact
661 gactctgtag accaggctgg ccttgaactc agagatctac ctgcctctgt ctctgcctct
721 tggtattaaa ggtatacatt accctgctca gcctgatgat tttttttaaa gagtttagaa
781 aaagataaat aaaaactaac tcaacacaaa gacatataag acagttacct atcacaggac
841 cc
//