LOCUS BG065514 609 bp mRNA linear EST 12-MAY-2010 DEFINITION H3032A04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3032A04 3', mRNA sequence. ACCESSION BG065514 VERSION BG065514.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 609) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG065514.1. Other_ESTs: H3032A04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3032 row: A column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..609 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3032A04-3" /db_xref="taxon:10090" /clone="H3032A04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 169 a 156 c 121 g 163 t ORIGIN 1 gcaaatatcc ttcatatatt cctacagagc actgtctatg gtgtggggct ttacctgcag 61 atcaccgcac ccacagtgcc ccagagctct cacacctagc ccagcagcct tggcttcagt 121 tcttaaaggc tccacattta ctggctttag caatgaactc ctttagcagg ggaccatcca 181 tttgccagaa cgcagccgtc tgggtaactt ctgtcaaatg attgatgcaa aacttaaagc 241 agaattcttc taaatcctct gcgtcatatc tgactgcagc agagaacaat gaaaaggcgt 301 tctccacagt aattcctcgc ttgatgatgt gctgacaaag cctcttcagt ctgttctcac 361 agtacgacgt tgccaagtcc aaaaggccta tagcatcctc aggaggcagg tccactgtgt 421 ctgtgtagag gtactggaga aaggcacggt acacagggta agaaaactgg tctatctcta 481 tcacttcctt catatcctca ttccaataag actgaaacat ggaccgaaag tgctcacacc 541 tgattttcag aacagcttta tggacatgaa tatattttcc atcaattcga aactttagat 601 cagcagttt //