LOCUS BG065322 829 bp mRNA linear EST 12-MAY-2010 DEFINITION H3029G06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3029G06 3', mRNA sequence. ACCESSION BG065322 VERSION BG065322.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 829) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG065322.1. Other_ESTs: H3029G06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3029 row: G column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..829 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3029G06-3" /db_xref="taxon:10090" /clone="H3029G06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 232 a 192 c 175 g 230 t ORIGIN 1 agtatttcat tatgctttat ttaaatacat tgaatatttc taggtgaaaa tgtagtatat 61 ggtttttcca aactgttttg aagtgagaaa atattttgct tttcaaccga taatacagat 121 atcacaaaat cattctatag tagtaagaac ttacaaaggc aaaaaaaatt accaagcctt 181 caaaacaagg taggagattc agttacagta ctacaaccaa ccactctaca gggcagtatc 241 acacatagtt taaaagttga aagactaaaa gctttgggaa gtatactaag ccataccaaa 301 ctggtatcta aatccccagg agacagactg atttcatctg gggaggtgac agaggatctt 361 gtagtccttg gagttctcgg ggaaggcagc gtgtcccagg cattgtaccc actgggtggt 421 ggggttggca tctgcacccc tgaccgctgg cagcagttct ctgggttgga catccaagct 481 tcaagcaact tctccctgtc ccaatcgtga gctcgaagca gtgcttcagc agtaaacaga 541 ggggcctgga gcatgtctgc agtctccaca ataagcatat cttttaacct acgaagatcc 601 tggggcctta gtccttcata tggctctcgc ttgtctaagg cagcatactc cgcttctatt 661 tcttcggcct ccgggtcccg agagaacacc atctgagact ccagactgag ggccaggtct 721 ttgtgctgct gcttttcggc acaatcacaa ggagtatctc tattttcatt ttcagcaaac 781 aagtctcctc catgttttac taagagctct acacaggctt tcatccccg //