LOCUS BG065226 707 bp mRNA linear EST 12-MAY-2010 DEFINITION H3028F10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3028F10 3', mRNA sequence. ACCESSION BG065226 VERSION BG065226.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 707) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG065226.1. Other_ESTs: H3028F10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3028 row: F column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..707 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3028F10-3" /db_xref="taxon:10090" /clone="H3028F10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 232 a 126 c 135 g 214 t ORIGIN 1 gtccattatg cttttaatgg agatttaaaa cttaatgcaa aagaaagttt tatatatcat 61 tagcaagtga ttaacttaaa ttatgtttta ataatgtgaa tacagtgtta gacctcaaaa 121 aagaaactac aaagttccct gcaacctaaa atgttggaaa ggctccttaa attgtcttta 181 cctaccaact ttataatact cagtggcatc ttgagggaca taagaaaata tgaggttctt 241 ttcagctatg atttggagct tcagtaaagc tgtatggtaa tgtcactatg ggaccataaa 301 attttcccaa aagtacttcc aatagcatag gcatttcaga ggcttaatat taatagttca 361 aagaatagaa agcaactaat ttatccttgg gaactgtaaa agataaatcc ttagagaagc 421 tttctagaat acagaactca aggatatcag ttctgtgaag tctcggtgag acactccaag 481 gcacagtgtt gagcacagcc tggtaatcaa gatgtcactg cactgtactt taggaggttc 541 aggatgctgt gcttgaagta acaggacatt gactttatcc ctagttaaga gcaccaggtg 601 tcaagctcag cttccattta cacaggatgc accaagaaat ccttaggatg accagtggtt 661 cgggtactgc attcctaaca ctttttacct ttctgcttta gggttca //