LOCUS BG064954 671 bp mRNA linear EST 12-MAY-2010
DEFINITION H3025G04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3025G04 3', mRNA sequence.
ACCESSION BG064954
VERSION BG064954.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 671)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG064954.1.
Other_ESTs: H3025G04-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3025 row: G column: 04
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..671
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3025G04-3"
/db_xref="taxon:10090"
/clone="H3025G04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 216 a 120 c 126 g 209 t
ORIGIN
1 ctttaacata gtcttatatt tggaaaagca gatttaaaaa acacttgaaa atacaagata
61 aggtaatggt tacttatgta agttttaacc ttatattgct tggccatttt tacatataaa
121 ttattgcttc ctactttgat aaatacacag cagagtcatg tacacagagg cagaaagcat
181 gaactatgag aaatcaaaca ctgtcaacgg cagtctggta actcaacaaa tgtttcatat
241 ttaccagcgc ttataatggt ggaaaactac gaggtgtagt ccctgagaag ttaggtagac
301 gcccggactg tgggttttct atcttctaat tgttatccca agctgacagc atcacggcgg
361 tcctaagcaa tgagacatcc aaaggcaaga gttcttgctt ctggtcagtg atttcatcct
421 ggtatttaat aacagtgtaa tacaaataca ataaataggc tatgcaaata aatattactc
481 tgctaaaaat gctcagtata tacagctttg ctttatacag tagtacattt cttctgactt
541 tttggcaatt ttcaaaatgt gttctctcta gagcaaaact ggcccactca ttactgagtg
601 ggctgaaatt tcaaggacat gtctttgaag ttgagaagaa attctatgct ggacagctat
661 agtggccctt g
//