LOCUS BG064934 733 bp mRNA linear EST 12-MAY-2010 DEFINITION H3025E05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3025E05 3', mRNA sequence. ACCESSION BG064934 VERSION BG064934.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 733) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064934.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3025 row: E column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..733 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3025E05-3" /db_xref="taxon:10090" /clone="H3025E05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 175 a 217 c 160 g 181 t ORIGIN 1 aaagtcaaaa tctttatcca agtcaccatc ctaacagtat tcttgaaaat gggctacctc 61 gtgtgagcac agatcacatc cgacctggct gcccagggca ctgtgctgcc ctctgtacac 121 acacactctc ctttccatcc tgctgtgccc acagacccag ctagtcaagg tccaaagcgt 181 ctctgggaca gacgtcctct ctgtccatca gcagcagaaa tacttgagtt caaacgatga 241 tacttgaagt tataggctca gcagctatct ctcagcttcc agagcggtga gaatacatcc 301 ttgcataaat atcgggagga gctacccaag aatgggcatc cacctgccag ctccgccttc 361 aaccagactt catctaagcc cagtttccct cagagaagtc accatttggg agaaaactgc 421 tcttgactgc agccctggtt cacaggaagg atgtcctctg catggagact cagcacatca 481 gagctgtaca gcaggtggga gggcctactt gttcctcttc cagtgtgcca ttgactcctc 541 catgaagttc tgaaatgctg gctcttcacg gctttcctct gtaactctgt ggtcaatgtc 601 atccgtgtca ctgtcagctt cttcatcctc ctcatccaag gttcctcgag tcaggatcaa 661 gtcagaagga tccagcacag gagatgcgtt gcctttgccg acctctggat ccagggctac 721 ggagcccata tcc //