LOCUS BG064922 598 bp mRNA linear EST 12-MAY-2010 DEFINITION H3025D03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3025D03 3', mRNA sequence. ACCESSION BG064922 VERSION BG064922.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 598) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064922.1. Other_ESTs: H3025D03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3025 row: D column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..598 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3025D03-3" /db_xref="taxon:10090" /clone="H3025D03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 158 a 167 c 146 g 127 t ORIGIN 1 actggaaagc aacttaaaat ttattctggc tcacagttaa ggtacagtcc ctgtggcaag 61 gagggcacag tagcaaaagt agctatggct gccgtagcag gcgagcggtg catctcatca 121 catccccagt gaggaaacag ctcaatttct ccattttccc ttttcaatca gtgggacccc 181 aggccacggt gccacccaca ttcagctggg acttcctgtc ccaggtaatc tctgggaacg 241 gtttcacagg cacacctgag atgtgtctcc tgggtgattc cacatcctgt caggctgaca 301 atgaagatga gtccacagag gatcagtgct gcctgctgtc agtgaccagc attatgtcca 361 atgcccatga acaaaaccag aaggagccaa tactcaggtg tccacacgac gatgtctcag 421 aagccacatc caatgtcggt gtgtaggttt gaaggagata cagcaaggca ccctgatgct 481 caccacacca catcttcggt gcactcagcc aagcactgag caaggtggct atccacatct 541 agctgggtca gcttcgggtc aaacgccttc tggcacaacg ggcaactcag caacgcaa //