LOCUS BG064831 758 bp mRNA linear EST 12-MAY-2010
DEFINITION H3024D01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3024D01 3', mRNA sequence.
ACCESSION BG064831
VERSION BG064831.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 758)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG064831.1.
Other_ESTs: H3024D01-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3024 row: D column: 01
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..758
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3024D01-3"
/db_xref="taxon:10090"
/clone="H3024D01"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 176 a 204 c 195 g 183 t
ORIGIN
1 cacactatga attgctttaa tggtggtatc tgtacaagtg atgtcaggac acaggacagc
61 aaatgcacag tggacatggc tagcagactg gctgtgaatg aataaagagt tcacactgct
121 cccatgcttt agtgactaag acagctctaa gccacctcct cctcagcctc ctcctcaaac
181 tctccctctt cctcagctgt agcgtcctgg tactgctggt actcggacac caggtcgttc
241 atgttgctct cagcctcagt gaactccatc tcatccatgc cttcacccgt gtaccagtgt
301 aggaaggcct tgcgtcggaa catggctgtg aactgctctg agatgcgttt gaacagctcc
361 tgaatagcgg tgctgttgcc aatgaaggtg gccgacattt tcaggccccg aggtggaatg
421 tcacagacag ctgtcttcac attgttgggg atccactcaa caaagtagct gctgttcttg
481 ttttggacat taagcatctg ttcgtccacc tccttcatag acatgcggcc cctgaacacg
541 gcagccacag tcaagtagcg cccatgtctt ggatcacagg cagccatcat gttcttggca
601 tcaaacatct gctgggtgag ctcaggaact gtcagggcac ggtactgctg gctgccccgg
661 ctggtcaagg gggcaaagca ggcatgaaga agtgcaggcg agggaagggc acatatttac
721 agccagtttc cgcagtcagc atttagctgg ccagggaa
//