LOCUS BG064801 735 bp mRNA linear EST 12-MAY-2010 DEFINITION H3024A04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3024A04 3', mRNA sequence. ACCESSION BG064801 VERSION BG064801.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 735) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064801.1. Other_ESTs: H3024A04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3024 row: A column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..735 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3024A04-3" /db_xref="taxon:10090" /clone="H3024A04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 189 a 194 c 187 g 165 t ORIGIN 1 gtttttgttt ttttgagaca gggttcctct gtatagacct ggctgtcccg gaactcactt 61 tgtagaccag gctggcctca aactcagaat tctgcctgcc tctgtctcct gagtgctggg 121 attaaaggca tgtgccacca cgcccggctg agttttactt tctaatgata gcccatctct 181 gatagcctct gttatttagg cacggtggac acagcacaca gaattcccca ggctttggtg 241 ggacaggaag gcagggcaga caggacagcc aacggggcct atggctaagt tggcctaagt 301 ttaataacaa agcagctttc tacccttccc ctaggcatca attccccaca gtggacttgg 361 gttagtccag cagggagatg aagatctgca atccagacag gaagtacata cccacacaca 421 cacaccaaac caacacagta tcaaaggacc aatatgacac aggagacaag accctctccc 481 ttcctctggt caaaatatcc aatggagatg ctggccgagc tcagtccatc caagaccctc 541 agtcactggg gcagggcgag ggacaagaag cagctgcctt taaatctaga tgttgtatag 601 cgtctgtagc agattgtggc gggcaaagga gcaggtttcc agcgcgccat tgggctttgt 661 cacaaatgcc aagagcaggg gtgcaagggc cttggggaga tagtcctgcc tcaccacgtg 721 gttcaacacc atcag //