LOCUS BG064798 737 bp mRNA linear EST 12-MAY-2010 DEFINITION H3024A01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3024A01 3', mRNA sequence. ACCESSION BG064798 VERSION BG064798.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 737) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064798.1. Other_ESTs: H3024A01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3024 row: A column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..737 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3024A01-3" /db_xref="taxon:10090" /clone="H3024A01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 164 a 182 c 204 g 187 t ORIGIN 1 gctttagaaa tgtggtttat ttccttgttg tcttgttact gtatacatag tatttttata 61 ccttaatgct tattcttgat ggcatctgtc aggtatttga agagatcaga aatgagattt 121 tttttcccct caaaagtcct ttattttctg atgagctcat caggcagttt tcaaaatcca 181 agtgtcttta ctgtctaaaa cactaccatt taagctatag acaaccgacc catggaaatg 241 caagccctgg tggtctgtcc tctgtgctgg aggacaggag aggacacagc tgcctgccct 301 ggggacaggt gtcggctgct gagcaaggct gactgcagtg ctgtggaacc cacagggagt 361 tcaccaagaa cagcaggacc agcaagctcg agcaaacaga gcgccttggc ctccctgtct 421 cctagccaaa gctgtcagcc ctctgtggcg tgtctgtggg aggtataaaa taaagctcct 481 gtcgtcacac agcaaggttc ccagggtggg ggtggaggtg ggggcggcgg cgagccacgt 541 tcgttcctgc agcatccact aggagcaaag gatcagggtc tcagagcccg ctgagaaccg 601 agggcttctg gggtcctgat gcttctgaca ccaccttgta gtagcttgtg agtggccagg 661 atccctgagg ccagcctgct gctaccagga gctgaagtcc ccaaagcccc gactgggcct 721 tttcttggcc gtggggg //