LOCUS BG064745 783 bp mRNA linear EST 12-MAY-2010
DEFINITION H3023D06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3023D06 3', mRNA sequence.
ACCESSION BG064745
VERSION BG064745.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 783)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG064745.1.
Other_ESTs: H3023D06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3023 row: D column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..783
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3023D06-3"
/db_xref="taxon:10090"
/clone="H3023D06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 224 a 206 c 165 g 188 t
ORIGIN
1 ccattttagt ggacatcttt attgtttaat agatcatcaa tttctgcaga cttacagctg
61 ggatttcatc agattgccat gctgagtcaa gaacagtgag tgacgaagct aaccagaggc
121 tacatacgtc agagagagag ctcagccttt acagctcact tcctttctca ggcagaatat
181 aaatagacgc cctctacaat gcacaatggt tttagtcact aaggaattta aatgggatct
241 tgaagaacac agacaaatcc tgatgcagta aagacgagct gagatgctgt gcaactgttt
301 aagggttcct ggtgccacat ctcagccact agctgaatct tgcgctaaca ccaaatggag
361 atgtggaaaa cactaggttg acttaggagc acaggaacca aaggcaggaa agaaaatact
421 aaacattgct gagagcatcc accccaggaa ggactttacc ttccaggagc tctagactgg
481 caccgccccc agtgctcaca tggctgactt tatcctctgt gttccatttg gcacagcaag
541 tggtagtgtc tccaccacct atgatagtga tgcaacccct agaagtggct ttcaccacct
601 catccatgag tgacttggtt cccctggcaa aggcttccca ttcaaatacc ccaacaggac
661 cattccaaac aatctgctta gctcgaccca cagcctcggc atatttcttg ctgctctcag
721 taccacagtc caagcccatc cagccagcag gtataccaga ggccacagta gcttggccag
781 tct
//