LOCUS BG064653 711 bp mRNA linear EST 12-MAY-2010 DEFINITION H3022C12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3022C12 3', mRNA sequence. ACCESSION BG064653 VERSION BG064653.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 711) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064653.1. Other_ESTs: H3022C12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3022 row: C column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..711 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3022C12-3" /db_xref="taxon:10090" /clone="H3022C12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 223 a 170 c 158 g 160 t ORIGIN 1 atataaaata tcagcaagcc gcaaaaaaaa aaaaaaaaaa attaaaaaaa agaaagaaaa 61 ttaaaaacca ggatttaggg aacaaaagga aaaccattgc tgaggtaact tcacaccttg 121 aggtagttta cttcgcacag cactgcctga ctccgcccac acgtcacggt agctttgtca 181 ctgttgtcct tgcaatatca ggaagcgggg accccgagca caggcagaga ggccttcctt 241 tcttcccagc acacttgaca gcattgtcca gggtagctaa agtgcatcac ctggctaata 301 tgcattacag tcagtacagg atcgggaggg aggctgagcc tcacttaact gatggccgct 361 gccctccaca agggttaact tccaacagga tctcagtgag cctggtaaat taataaatta 421 agtgtgctca cccctctgtg cataatgcgg ctggtactct ggtttgtttt caggcacacc 481 tttcataata acaacgacat tcacggaggc ataagcagtc agttaaatac tcctctagat 541 aaaaacagaa gcgacattct ccaatttact aagtttgcca gatgatggac taaattttaa 601 gctttatcag gagctcaggg ttgggttgta gtccgaccac cacaagggaa cagtgccgtg 661 gtggggacac acacaccgca acagctgcct cagccttgtc aagcacgcgg g //