LOCUS BG064558 502 bp mRNA linear EST 26-JAN-2001 DEFINITION H3021B09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3021B09 3', mRNA sequence. ACCESSION BG064558 VERSION BG064558.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 502) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3021B09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3021 row: B column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..502 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3021B09-3" /db_xref="taxon:10090" /clone="H3021B09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 141 a 84 c 97 g 180 t ORIGIN 1 gtgtttgtgg tttttttttt tttttttttt tttttttttt ttttttttat tttttttttt 61 tttttttttt gctttgacca agtttggttt attttatccc tctggttact tctgccaacc 121 tcattatggt tttacatatt tgggcttttg aaacggagaa acccagcacc cactgggacc 181 agggggcatc tggatgggta aatcaaaagg cactgggcac atgacgggta cccttttttt 241 tgggactcta ggctcagctc attaactttg ctagcaggga tgaatctcaa ctcactgcca 301 agttcaaggg ccttggaaaa ctggcgataa aggtgaaaca gttagaaata ttgatacttg 361 gaaaaaaata gggctttctt tttggcttat gaaaaggtaa aaattgaaat ggaaacccag 421 aaatatggct cgcattggtt tggaaaaaaa caaaacccaa aacccaaagc tgttagaaaa 481 caaacccatt aaaaaatatc ct //