LOCUS BG064537 782 bp mRNA linear EST 12-MAY-2010
DEFINITION H3020H06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3020H06 3', mRNA sequence.
ACCESSION BG064537
VERSION BG064537.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 782)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG064537.1.
Other_ESTs: H3020H06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3020 row: H column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..782
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3020H06-3"
/db_xref="taxon:10090"
/clone="H3020H06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 161 a 225 c 216 g 180 t
ORIGIN
1 cttttttggt aataaaaatt ttcttggttt aggcgaaata ctctgtgcaa cgcagtaccg
61 agggggagcc ctgccccccc ccagccccct ccccttcctt tgcctcctgc aggggggaag
121 ggagggactt ctgggcaggg gtgaatgtgg gagggagaag aaccacttcc catcccccac
181 aaacatggaa gaaaaaaact gagaagggcc tggaagggga caagtgccag caggggagac
241 acaccaattc atcctgcagt ttctcccagg gggatgcagg gttggcccaa gcagccaagt
301 ccactggtgg gcctctgctg cccctcctga cagcctgtta aagcttcagc tcattggcta
361 ttttggaggc aatgtttttg aaggccatgg aagtgcccga tatccgctta aaccgaacac
421 cgttgagaga cagccggggc agtttgcaca cctccatctc ccactgcaca aagttctcgt
481 ggcctggtgt gccatgcagc acagtagcat gtaccgctcg tgcagctcgc tttggcagct
541 gttggcgtcc agcaccttgc ggatctcccg catcatctca ttgggctcca tagagctcgt
601 ggtcttcatg ctccaggtga agcgcaggga gcgaggcttg gctcccgaaa ctcctccttg
661 tccttgtcag tgcctccact gcctaccacg tgaggtctga gcgtctccac tcggtctttg
721 ctttcaggtt cattcaggtt cctgcggaca aacttggagg tgaacttgct gaagatgctg
781 cc
//