LOCUS BG064472 804 bp mRNA linear EST 12-MAY-2010
DEFINITION H3020B06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3020B06 3', mRNA sequence.
ACCESSION BG064472
VERSION BG064472.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 804)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG064472.1.
Other_ESTs: H3020B06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3020 row: B column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..804
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3020B06-3"
/db_xref="taxon:10090"
/clone="H3020B06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 233 a 196 c 159 g 216 t
ORIGIN
1 atttaatttt tttttatttt cctccacact ggcaaaagtt ccgagggagc ttaaagtttt
61 gtaaacattt taactatccc tcttccccac cccccctttt gaatttacaa agcaaaggag
121 aggagtctca cattttaatg gttcctatct ccttatgcta tttgatccaa aaactattta
181 caagtttgta gcagtctctg tatagttatt gtacacattt aggagggaga gaggcaagaa
241 aggataggga gaatggtgat gcaaaataat aaaaatagaa accaaatata atcttccaat
301 accaaactgc tctgagagca actccagcct tgtccaggca cagatgccga gctgcccacc
361 acagcacctc ttgcacaggc ccagctgacc cacagctgcc gccgaggtgt tcgactatcc
421 caggtgatgt tcactaggcg aggagcagaa gcagtcagag tctgtctgta cagagcttga
481 gtacacttgg ccttgctatg gagcaggcct cccactcctg tgcctgccat ccccataccc
541 cattgcatcc tcctgcatag tggtttccac ggatgaaggt cttaaagatg gtcaggcttt
601 gtatgttctc ttttcagaaa aggcaaacaa cttgccatcc cagaatcttt tcacaagacc
661 ttttcagggc catggcaaaa catgcacacg gtgctctgag gaacagatga aattcccagc
721 ttttatcaga agcaaaagtt tatccctatc cgagttgaaa ttacacaaat tcatacatat
781 ggtaaagatg cctcttaaac gaga
//