LOCUS BG064472 804 bp mRNA linear EST 12-MAY-2010 DEFINITION H3020B06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3020B06 3', mRNA sequence. ACCESSION BG064472 VERSION BG064472.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 804) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064472.1. Other_ESTs: H3020B06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3020 row: B column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..804 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3020B06-3" /db_xref="taxon:10090" /clone="H3020B06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 233 a 196 c 159 g 216 t ORIGIN 1 atttaatttt tttttatttt cctccacact ggcaaaagtt ccgagggagc ttaaagtttt 61 gtaaacattt taactatccc tcttccccac cccccctttt gaatttacaa agcaaaggag 121 aggagtctca cattttaatg gttcctatct ccttatgcta tttgatccaa aaactattta 181 caagtttgta gcagtctctg tatagttatt gtacacattt aggagggaga gaggcaagaa 241 aggataggga gaatggtgat gcaaaataat aaaaatagaa accaaatata atcttccaat 301 accaaactgc tctgagagca actccagcct tgtccaggca cagatgccga gctgcccacc 361 acagcacctc ttgcacaggc ccagctgacc cacagctgcc gccgaggtgt tcgactatcc 421 caggtgatgt tcactaggcg aggagcagaa gcagtcagag tctgtctgta cagagcttga 481 gtacacttgg ccttgctatg gagcaggcct cccactcctg tgcctgccat ccccataccc 541 cattgcatcc tcctgcatag tggtttccac ggatgaaggt cttaaagatg gtcaggcttt 601 gtatgttctc ttttcagaaa aggcaaacaa cttgccatcc cagaatcttt tcacaagacc 661 ttttcagggc catggcaaaa catgcacacg gtgctctgag gaacagatga aattcccagc 721 ttttatcaga agcaaaagtt tatccctatc cgagttgaaa ttacacaaat tcatacatat 781 ggtaaagatg cctcttaaac gaga //