LOCUS BG064071 709 bp mRNA linear EST 12-MAY-2010 DEFINITION H3015B10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3015B10 3', mRNA sequence. ACCESSION BG064071 VERSION BG064071.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 709) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064071.1. Other_ESTs: H3015B10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3015 row: B column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..709 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3015B10-3" /db_xref="taxon:10090" /clone="H3015B10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 175 a 158 c 183 g 193 t ORIGIN 1 gcttgtcctt ttatttattt agtttttttt aaatcttact gtgcagagat taggagttac 61 aaggaggaag gaagtgaccc aatgttttga gtgatgtcac tttagacaag accccacctg 121 tcattggtcc tcacttcctt gcaaaggaag acccaccaga aaggtgtcag cttgggcagt 181 ctgagtctta tcttctgagg ataatgaggc aggggagggc taaaagatgc ttgaagcctt 241 tttctcctta gggatttttt ttcctcttgg gaattgagat aaaaatggac ccagcctagc 301 acagagcctc ccgccgggat tcctcaccga tgtcttgctc cacattagtg atcacagtga 361 tatcatcgat gtaagtggta ggggtggtat agaggaccac agatcgatgc agtcccgcat 421 agttgaagaa gtcaaagctt gtgtcctgga caaagtaacc cttgggatac atggaggtgt 481 cagtcttgta gacgatggtc cccggcggaa gggtatgagg ggtcagtgtg ttgttgatgg 541 caatcgtgat ccggcaggtg gtcaggggcc cactctggac cagcttgcta atgtcagcct 601 caaaggggag gtgacctccc tcatgttcca ccacatgaat cccattcacc cacacaactg 661 cataataatg ggcactgttg atcctcaaca ctactctcat gtcggtatc //