LOCUS       BG064040                 529 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3014H01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3014H01 3', mRNA sequence.
ACCESSION   BG064040
VERSION     BG064040.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG064040.1.
            Other_ESTs: H3014H01-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3014  row: H  column: 01
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3014H01-3"
                     /db_xref="taxon:10090"
                     /clone="H3014H01"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          148 a          134 c          110 g          137 t
ORIGIN      
        1 gatgcatgca gtggaacttt aatttgattt gaaactacaa taaaagttgc ctttcaaaat
       61 ttaattagat ttggatgaca aagtctcctc tcttctgcca tggcagatct gcattttcaa
      121 tgggacagta aagctctctc ttctgacgcc ttccttcctt taggcttctt cccttttgat
      181 ttctgtttct tcaaaacctt caacatcctg ttcatcctct tcctcctcca gcacatggag
      241 cagcagcaca taggcagaaa tcccaggact agaatgctga tcgcagcaag gccaaacacc
      301 cgtagcacct gtgtaggctt tgttggaaag aaaaagtcca acatatttcc caaagggggt
      361 tcaagttctg cagcttcagc aagaatgggc aatttcgcag tgttcacagg acggaacagg
      421 atcaccagcg tccagaataa aagtagaagc aggcccaagt gcctcctggc ccacaagtat
      481 tgcagacact ggcttactga agtccagaag ccaaaccgag gggcatccg
//