LOCUS BG063939 573 bp mRNA linear EST 12-MAY-2010 DEFINITION H3013F03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3013F03 3', mRNA sequence. ACCESSION BG063939 VERSION BG063939.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 573) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG063939.1. Other_ESTs: H3013F03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3013 row: F column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..573 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3013F03-3" /db_xref="taxon:10090" /clone="H3013F03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 155 a 125 c 121 g 172 t ORIGIN 1 cacaatgaac acaccacttt attttaggga cccaaaaccc aaagataatt taccatatgt 61 gcatggtttg ctctaatatt tatctgtgtt atgtgtacct ggtcccagca aagacctgaa 121 aagaatgcgt gttttgtttt ttttggtttt tggagacaag ggtttctctg tgtattcctg 181 gctgtcctgg aactcactct gtagatcagg ctggcctcag aaatccgcct gcctctgcct 241 cccaagtgct gggattaaag gcgtgcacca ccacgcccgg ctgtttttgt tttttaattg 301 aagttacagc taccacgtgg gcattgagac gtgaaccttc tacaattgaa tctttatagc 361 tgatccatct ggacagcccc taaagatcgc ctttacttaa cctggaaagt gaagaagttc 421 tgcaaggtca tgaaggtcat gccctgaact gaaatttaag tttccttctg caaagctgat 481 attggaaaag gtgtcaagaa gaccagctct cagagcagag actttaatga atatatttta 541 caaatacact ggagaatcat gcaatgctgc ctg //