LOCUS BG063761 674 bp mRNA linear EST 12-MAY-2010 DEFINITION H3012E01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3012E01 3', mRNA sequence. ACCESSION BG063761 VERSION BG063761.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 674) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG063761.1. Other_ESTs: H3012E01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3012 row: E column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..674 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3012E01-3" /db_xref="taxon:10090" /clone="H3012E01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 182 a 170 c 155 g 167 t ORIGIN 1 gccaatgtta atgagtgttt attgttgata aaaccatagc acatttacag taagtaacaa 61 cattaaggta tagttacagt attatacaat attacagcaa taaaatacca ttcattcctt 121 tgtacatccc gcacaagcag caagttgaaa atatctttcc aggattgcaa gccctggggg 181 cttctgatga ataaatagtt tctaaggtag ctgttcttga tgctgtccca gcatctgtcc 241 acaaacaggc cagaggatgg ggagccaaga ctgaggacag gatgggaggg gacatagctt 301 ctcttccacc gaggccttgt ccttcatgcc ccagagtcac agatcccagg tgacaagtgt 361 gctcttgcac ccctgtccta actggaaggc actctgctgc ctcagcctgc agacacatta 421 gagctaggcc cgacctagaa ccaacctctt cactttggct gccagttgta gcagcaagag 481 cggaagtcct tgagttcctg tactgtagcc catcatcagc tcggaagctc tgagctcaga 541 ctcctaacat agtagtctcg tggggcaggc gactagaggt ccagtcttca cagacagggg 601 ccacctcacc agccctttca aaggcacagc ttggaggaca ggatttggaa accctttctt 661 ctctgtttag gaaa //