LOCUS BG063682 565 bp mRNA linear EST 12-MAY-2010 DEFINITION H3010C05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3010C05 3', mRNA sequence. ACCESSION BG063682 VERSION BG063682.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 565) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG063682.1. Other_ESTs: H3010C05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3010 row: C column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..565 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3010C05-3" /db_xref="taxon:10090" /clone="H3010C05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 173 a 127 c 150 g 115 t ORIGIN 1 cagaaatcga aaaagatgta ttaaaatatt cacagacgta caaatgaaaa tatcaaaaag 61 taacaatgaa cttggtatct ctccagctgg acacatttcc aggagaaaat acaggcagga 121 attctaaaag ccatcccttt taggtgtctc ttctcaattc gaatgcattc ttctgctcag 181 aagacacagg aagcacaggt ctgccatctg gacgtggcct cactgttatc tgtagccagg 241 cccagaacgc agagttgcag gaaagagacc gaatactcca agggaggtgg tgtgtggagt 301 taggccatga ggctgcacac tgtcctgcga gcagctggaa ccaggctgca ggctcagtcc 361 aggaaggccg gccagcagcc aacggtttct gcctagggaa gaaggttggc ttggccacag 421 aggatttggt tctgactggg cagggagatc atggagccag atgtagaaag cccttaagaa 481 gaggtgtcat ttgaacaaag cccacagagc actagcacca tatacacggt ctcagcacca 541 caaggagatg gtaaagaacc tggtg //