LOCUS BG063496 744 bp mRNA linear EST 12-MAY-2010
DEFINITION H3007H09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3007H09 3', mRNA sequence.
ACCESSION BG063496
VERSION BG063496.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 744)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG063496.1.
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3007 row: H column: 09
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..744
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3007H09-3"
/db_xref="taxon:10090"
/clone="H3007H09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 288 a 141 c 108 g 207 t
ORIGIN
1 gtaaaactta ttaaataatt ttattatttc ctccttcata ttgctaaata gtagcccaag
61 tgtaagcatg ttagaaaatc tgaaggaata aaacacattt tggtttacat aaagcatata
121 taaaaatatc tatttagaaa acaaacatct gataattaca ctgcccaacc atctttaaga
181 gtgctaaaaa acaaaacaaa acaaaatgat ataataaaat ccctgcaaat caaatgaggc
241 aagatagttt tgctaaagtt ttgttcattt tggcatttta ggaaactgac ctgtactaat
301 gaaatttaag tcaaagttat ggccaggtaa taggagtaaa aatatacagc cacttaaagg
361 cattcttcaa acaaaacctg ctttgtgctt agaacagtta acagcactca acattacaaa
421 acttcccaac tttgctagta accgttctac aatagcaaga ggtatcaaat ggaagtgtca
481 gtatacaaag cacttattta aaaaaaacaa aacagtaaga tccatggtta attaccttct
541 gattctctta aaacccccaa agcataaaat gtttgcttac tgctcagtag ctttaagcca
601 taaccaaaaa cccaaatagc tctgtttatc aagtagaaga accgtgtgaa atgctagtca
661 ttacaacagc gtgaagtgct acaagtttgg atagccactg ctcaccacaa tctgtcactg
721 cagcctgatg acgaaccaac tcag
//