LOCUS BG063426 663 bp mRNA linear EST 12-MAY-2010 DEFINITION H3007B05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3007B05 3', mRNA sequence. ACCESSION BG063426 VERSION BG063426.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 663) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG063426.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3007 row: B column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..663 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3007B05-3" /db_xref="taxon:10090" /clone="H3007B05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 121 a 203 c 176 g 163 t ORIGIN 1 gccgatgcag ctctctcaga gaggcccttt attaaagcca acagcagttt cagactatct 61 tccaggctcg ggacagggac atgagatgga caaaagggta tggggggtct ctccaagccc 121 acagcttggt tgtatgtgct caattgtggg tgcccctgcg ggtagtagat gtggtcttga 181 tggaataggc cctgagggct ccgggtcccc cactgaagct cagagcattg cttcccatgg 241 ttcctccgaa gataagcttg ccaccattgc ccccagtgga attcacaaca gagatattca 301 caggtcccat tccgtctcca gacaaccggc tctcctcgcc ctccagcagc ttgcggtagg 361 tggcgatctc aatgtccagc gccagcttgg tgttcaggag ctcctggtac tctcgaagtt 421 gccgggccac atcctgcttg gccttctgca gggcagcctc cagctccccc tgcttagcat 481 gagcatcctt gattgccagc tccccttgtt cctcagcctc tgcgatgctg gactctaact 541 tggcacgctg gttcttcaag gtgtcaatct ctgcctgcag cctctggata gagcggttca 601 tctccgcaat ctcattccgg gtgttgcgga ggtcatcccc gtgcttccca gcctgggcct 661 gga //