LOCUS       BG063412                 611 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3007A01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3007A01 3', mRNA sequence.
ACCESSION   BG063412
VERSION     BG063412.1
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished
COMMENT     Other_ESTs: H3007A01-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3007  row: A  column: 01
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3007A01-3"
                     /db_xref="taxon:10090"
                     /clone="H3007A01"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          220 a          103 c           90 g          198 t
ORIGIN      
        1 ctttttgaac taatggtgcc caaataacca aaaacacaca gataaaacat cctatacttt
       61 atctcatttt tttaacacaa aactaaaact taattatttt ttgagctaca atttttgata
      121 ggagtactgt tttatataaa aagaaactga atttgaacag gtagagttat acaaagtggt
      181 tccacaaaat ggttcaaaga gaattttcta attgcagaat gcttctgttt agcgtgtggt
      241 ggtgtcccta gcatgtgcag tacatatcgc tcttgtagtc ttacaagtct cactagtgaa
      301 aggcaactca ctcatacacc ttagcaagaa cacacagact gttttgaagt atgtaactaa
      361 tcttcataga gatgcataca agttttggtt caactcttac aaattaaaag attcactggg
      421 agaatttttg tacaataaag acttcacaca acgtacaata taaaatgtaa attaattcac
      481 taaaaaactg aaatgcatag actctgcata ggtgaaccgg tagctctttt aaatgtcttt
      541 cttttctatg gtaaatatat atttcattta cttaatggaa tcacagcaaa atacaactat
      601 agtttcaaag c
//