LOCUS BG063228 566 bp mRNA linear EST 12-MAY-2010 DEFINITION H3004F04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3004F04 3', mRNA sequence. ACCESSION BG063228 VERSION BG063228.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 566) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG063228.1. Other_ESTs: H3004F04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3004 row: F column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..566 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3004F04-3" /db_xref="taxon:10090" /clone="H3004F04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 106 a 195 c 118 g 147 t ORIGIN 1 cttagagcac acagcccagt tttattatga cagaagcaca cagcctgtgg gcctcaaatt 61 cacatcccac tctaaccctc gttccacgtg gacagaagcc actgtgaagt caggctagcc 121 cccgtcagac tgtgggcacc caaggctcac agaagaccac tctgcagggc ctcctcactc 181 ctggctcgac accagtgatg gtgaaaccat tgcgcagtct agtgtctggc ctgtgcaatg 241 tcttgaactg catcatcaaa gcatgatttt gccatggcct gggctacttt caacactttc 301 ttctcctttg cttcaggccc cacatggctc cgggccagct tcagccagtg ctgctcagtc 361 cgggacaggt actcttcgaa ttgctcccca cgggccacct ctgggtgctg cggatctgct 421 ccctcctcag cactgtcctc ctggtcttca tcctctccat ctgcactctc ttcatctgag 481 ctgctgtcct cttctgagtc cgactcctcc agccactcct gggatttcgg atccatctcc 541 accaaggttt tccactcttc catctt //