LOCUS BG063217 577 bp mRNA linear EST 26-JAN-2001 DEFINITION H3004E01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3004E01 3', mRNA sequence. ACCESSION BG063217 VERSION BG063217.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 577) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3004E01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3004 row: E column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..577 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3004E01-3" /db_xref="taxon:10090" /clone="H3004E01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 210 a 106 c 85 g 176 t ORIGIN 1 actagagaag gaggtgcaat tcagctttat tttcacatca tttctatata cattatatat 61 gtcataaata aacacttgta gttaaaaagt ccatataaaa cccaagtata caaaggtggt 121 aggagacaga catactgcag tgagcttatg atactttgtt gtttaaatac ttttaaaata 181 agttttcaaa aagacaacaa atcaaaagca attacatttt cagagagctc catgtttaca 241 gaagtttatc attcaagtac gttttattaa aataataacc acgttactgg ctgcaaagca 301 caggaagaga gcccggatac tgagcgagac cagagctgtc ttagcatgtt attccttagc 361 ctagaaccca cttctaacat gaactgcaga gcatgcccat gattttgtct ttacaccata 421 aaacactgtg ctgacaactc atcctataca tttccttaga tacttattta aaataaatac 481 taaacaattc ggttaataaa tactacaaat aacaatgatt tgatttataa aatagtacat 541 ctctttcaat gtgcttggca actggacatc atacctg //