LOCUS BG062955 582 bp mRNA linear EST 12-MAY-2010 DEFINITION H3002D08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002D08 3', mRNA sequence. ACCESSION BG062955 VERSION BG062955.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 582) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG062955.1. Other_ESTs: H3002D08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3002 row: D column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..582 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3002D08-3" /db_xref="taxon:10090" /clone="H3002D08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 112 a 159 c 162 g 149 t ORIGIN 1 ctctttgaaa atttttcctc tttatttagg tttttctttt tgttgagaga gggtctcact 61 atttagccca ggatggcctt gaactcttga ccctcccact tctgtctccc aagtgctggg 121 attacaaccc attctaccag gcctggccag tctcctggag tgagggccca aacaactgag 181 tgttgggcag aaccaggcca gcctgagagg gtcaagcaga ggtgtccagg gactctgtct 241 cactgtgtac atcccgtgac cacagagctg agtcaggatg gtcagagcag gtgggcggag 301 agccatttcc caccccagct gttgagccca gctcctttaa gggggcagta gtgcaggcca 361 cactccagca cactggcctt ccaagggctc tgtccccagc agagctccag cctcagactg 421 atgtcttgca gtgacctgac cccagggggc ctctcaggct ttggactggc agcttttggg 481 gtcttcaggc ttctctgcag gaggggttga ggtcagtgag gcgtccaggg tttgtctccg 541 gataagctct gaatagagcc cgcctttttt aaggagttct tc //