LOCUS BC085015 1161 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens eukaryotic translation initiation factor 5A, mRNA
(cDNA clone MGC:99547 IMAGE:6575132), complete cds.
ACCESSION BC085015
VERSION BC085015.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1161)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1161)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (25-OCT-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 59 Row: n Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34147551.
FEATURES Location/Qualifiers
source 1..1161
/db_xref="H-InvDB:HIT000266102"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:99547 IMAGE:6575132"
/tissue_type="Prostate, carcinoma"
/clone_lib="NIH_MGC_40"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1161
/gene="EIF5A"
/gene_synonym="EIF-5A"
/gene_synonym="EIF5A1"
/gene_synonym="uORF"
/gene_synonym="uORF A"
/db_xref="GeneID:1984"
/db_xref="HGNC:HGNC:3300"
/db_xref="MIM:600187"
CDS 33..497
/gene="EIF5A"
/gene_synonym="EIF-5A"
/gene_synonym="EIF5A1"
/gene_synonym="uORF"
/gene_synonym="uORF A"
/codon_start=1
/product="eukaryotic translation initiation factor 5A"
/protein_id="AAH85015.1"
/db_xref="GeneID:1984"
/db_xref="HGNC:HGNC:3300"
/db_xref="MIM:600187"
/translation="MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMS
TSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLL
QDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK"
BASE COUNT 266 a 318 c 304 g 273 t
ORIGIN
1 gttcgcgcga gttggaatcg aagcctctta aaatggcaga tgacttggac ttcgagacag
61 gagatgcagg ggcctcagcc accttcccaa tgcagtgctc agcattacgt aagaatggct
121 ttgtggtgct caaaggccgg ccatgtaaga tcgtcgagat gtctacttcg aagactggca
181 agcacggcca cgccaaggtc catctggttg gtattgacat ctttactggg aagaaatatg
241 aagatatctg cccgtcaact cataatatgg atgtccccaa catcaaaagg aatgacttcc
301 agctgattgg catccaggat gggtacctat cactgctcca ggacagcggg gaggtacgag
361 aggaccttcg tctccctgag ggagaccttg gcaaggagat tgagcagaag tacgactgtg
421 gagaagagat cctgatcacg gtgctgtctg ccatgacaga ggaggcagct gttgcaatca
481 aggccatggc aaaataactg gctcccaggg tggcggtggt ggcagcagtg atcctctgaa
541 cctgcagagg ccccctcccc gagcctggcc tggctctggc ccggtcctaa gctggactcc
601 tcctacacaa tttatttgac gttttatttt ggttttcccc accccctcaa tctgtcgggg
661 agcccctgcc cttcacctag ctcccttggc caggagcgag cgaagctgtg gccttggtga
721 agctgccctc ctcttctccc ctcacactac agccctggtg ggggagaagg gggtgggtgc
781 tgcttgtggt ttagtctttt tttttttttt tttttaattc aatctggaat cagaaagcgg
841 tggattctgg caaatggtcc ttgtgccctc cccactcatc cctggtctgg tcccctgttg
901 cctatagccc tttaccctga gcaccacccc aacagactgg ggaccagccc cctcgcctgc
961 ctgtgtctct ccccaaaccc ctttagatgg ggagggaaga ggaggagagg ggaggggacc
1021 tgccccctcc tcaggcatct gggagggccc tgcccccatg ggctttaccc ttccctgcgg
1081 gctctctccc cgacacattt gttaaaatca aacctgaata aaactacaag tttaatatga
1141 aaaaaaaaaa aaaaaaaaaa a
//