LOCUS BC084582 1912 bp mRNA linear HUM 20-JUL-2007
DEFINITION Homo sapiens LAG1 homolog, ceramide synthase 1, mRNA (cDNA clone
MGC:90349 IMAGE:6192817), complete cds.
ACCESSION BC084582
VERSION BC084582.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1912)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1912)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-OCT-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. James R. Lupski
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 174 Row: n Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 11641419.
FEATURES Location/Qualifiers
source 1..1912
/db_xref="H-InvDB:HIT000266090"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:90349 IMAGE:6192817"
/tissue_type="Peripheral Nervous System, sympathetic
trunk"
/clone_lib="Lupski_sympathetic_trunk"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1912
/gene="LASS1"
/gene_synonym="CerS1"
/gene_synonym="LAG1"
/gene_synonym="UOG1"
/db_xref="GeneID:10715"
/db_xref="HGNC:HGNC:14253"
/db_xref="MIM:606919"
CDS 165..884
/gene="LASS1"
/gene_synonym="CerS1"
/gene_synonym="LAG1"
/gene_synonym="UOG1"
/codon_start=1
/product="LASS1 protein"
/protein_id="AAH84582.1"
/db_xref="GeneID:10715"
/db_xref="HGNC:HGNC:14253"
/db_xref="MIM:606919"
/translation="MPESAWKFLFYLGSWSYSAYLLFGTDYPFFHDPPSVFYDWTPGM
AVPRDIAAAYLLQGSFYGHSIYATLYMDTWRKDSVVMLLHHVVTLILIVSSYAFRYHN
VGILVLFLHDISDVQLEFTKLNIYFKSRGGSYHRLHALAADLGCLSFGFSWFWFRLYW
FPLKVLYATSHCSLRTVPDIPFYFFFNALLLLLTLMNLYWFLYIVAFAAKVLTGQVHE
LKDLREYDTAEAQSLKPSKAE"
BASE COUNT 399 a 546 c 614 g 353 t
ORIGIN
1 gagaatgttt cattgagcct acgcctcctg aatgcgggag ggccacggga tggtcccaag
61 actgtgccaa ggcgtgccag gcctcgggtc cttgagaagg agcgagggct ccaagaacgc
121 ccctggcgaa gcggtgctgc ctccagccca gagatgccgc caagatgccc gagagcgctt
181 ggaagtttct cttctacctg ggcagctgga gctacagtgc ctacctgctg tttggcaccg
241 actacccctt cttccatgac ccaccatctg tcttctacga ctggacgccg ggcatggcag
301 tgccacggga cattgcagcc gcctacctgc tccagggaag cttctatggc cactccatct
361 acgctacgct atacatggac acctggcgca aggactcggt ggtcatgctg ctccaccacg
421 tggtcactct catcctcatc gtctcctcct acgccttccg gtaccacaat gtgggcatcc
481 ttgtgctctt cctgcacgat atcagtgacg tgcagcttga gttcaccaag ctcaacattt
541 acttcaagtc ccgcggcggc tcctaccatc ggctgcatgc cttggcagca gacttgggct
601 gcctcagctt cggcttcagc tggttctggt tccgcctcta ctggttcccg ctcaaggtcc
661 tgtatgccac cagtcactgc agtctgcgca cggtgcctga catccccttc tacttcttct
721 tcaatgcgct cctgctgctg ctcaccctta tgaacctcta ctggttcctg tacatcgtgg
781 cgtttgcagc caaggtgttg acaggccagg tgcacgagct gaaggacctg cgggagtatg
841 acacagccga ggcccagagc ctgaagccca gcaaagccga gtgagtgtgg aggggcccgc
901 accccgcgtt ccctccttta gtgtcacccg tccaagcgtg ttcgtgggca ggcccccaag
961 acccagcagc agatgaggcc gacgcggatg ctgcccccca agcctactcc aaaacagaga
1021 ctgcaataaa ggcgtggacg atgtggccag taccctgaag gacaggacag tgaggaaggg
1081 gagggagagg ctggggaggg gcaggcaggg cgacaggcag ggtctcagcg gtagggacac
1141 tgagggccag gcctagtggc tggcgtggcg ggagtggcga gccagggccc tgtgggtggt
1201 gaggagtagt tgctggccag cctggatgac gacctctgac ccatgtcgcc actggagctg
1261 gtggcagtgg ggctggggag gaaggaacgc caagggccgc agagagaacc caggctccat
1321 ctgggccgag acatcctggc ctctgagtct gacaggggag cccactgccc ggccaaacag
1381 gagctggggc tgggagctca gactcagtcc agcccaggtg ggagtcctgg ggaaggagat
1441 agcccacgag cctcaccagc cctgggtgac agccagatgg tgtccgaagc cccaggcctg
1501 gggcaggcag ggggtgtctg ggccagggat gaacggaggc caaactgggt aacaagcaga
1561 gtcgggtgtg cagtgtctca taggcaacgt gaggagtgtt tgagcaggag ccggggcagg
1621 aggttgtgga aggccctgga aggtgccgac cagccaggcc ctctgatttg gccggtgggg
1681 gccatcggtg acgtgggaac gatggtttca gggaagcagt ggctcagagc tccgtgacgt
1741 tttttggaac gggaggtgcg agatgcagac atttaacaca tccggggctg tagccaggca
1801 gaggagcgtc agacccccca ccctgtttgg aagggagaac ccatcagatt cgccttgggt
1861 gtagatgaaa cagaaataaa agaatcaaag acgactcaaa aaaaaaaaaa aa
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