LOCUS BC083514 3421 bp mRNA linear SYN 02-SEP-2016
DEFINITION Synthetic construct Homo sapiens
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble), mRNA
(cDNA clone MGC:90332 IMAGE:4443624), complete cds.
ACCESSION BC083514
VERSION BC083514.1
KEYWORDS MGC.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 3421)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3421)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-OCT-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 166 Row: d Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 54020719.
FEATURES Location/Qualifiers
source 1..3421
/db_xref="H-InvDB:HIT000266044"
/organism="synthetic construct"
/mol_type="mRNA"
/isolation_source="Homo sapiens; Liver, adenocarcinoma"
/db_xref="taxon:32630"
/clone="MGC:90332 IMAGE:4443624"
/clone_lib="NIH_MGC_90"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3421
/gene="HMGCS1"
/db_xref="GeneID:3157"
/db_xref="HGNC:HGNC:5007"
/db_xref="MIM:142940"
CDS 66..1628
/gene="HMGCS1"
/codon_start=1
/transl_table=11
/product="3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1
(soluble)"
/protein_id="AAH83514.1"
/db_xref="GeneID:3157"
/db_xref="HGNC:HGNC:5007"
/db_xref="MIM:142940"
/translation="MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDA
GKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSK
SVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDI
AVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDG
KLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLA
RMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKAS
LLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDA
TPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTYHLVNYIPQGSIDSLFE
GTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAA
EPEAAVISNGEH"
BASE COUNT 1076 a 564 c 758 g 1023 t
ORIGIN
1 cggacgcgtg ggtcgaccca cgcgtccgtc ctctgctgcc gctcggtcac gcttgctctt
61 tcaccatgcc tggatcactt cctttgaatg cagaagcttg ctggccaaaa gatgtgggaa
121 ttgttgccct tgagatctat tttccttctc aatatgttga tcaagcagag ttggaaaaat
181 atgatggtgt agatgctgga aagtatacca ttggcttggg ccaggccaag atgggcttct
241 gcacagatag agaagatatt aactctcttt gcatgactgt ggttcagaat cttatggaga
301 gaaataacct ttcctatgat tgcattgggc ggctggaagt tggaacagag acaatcatcg
361 acaaatcaaa gtctgtgaag actaatttga tgcagctgtt tgaagagtct gggaatacag
421 atatagaagg aatcgacaca actaatgcat gctatggagg cacagctgct gtcttcaatg
481 ctgttaactg gattgagtcc agctcttggg atggacggta tgccctggta gttgcaggag
541 atattgctgt atatgccaca ggaaatgcta gacctacagg tggagttgga gcagtagctc
601 tgctaattgg gccaaatgct cctttaattt ttgaacgagg gcttcgtggg acacatatgc
661 aacatgccta tgatttttac aagcctgata tgctatctga atatcctata gtagatggaa
721 aactctccat acagtgctac ctcagtgcat tagaccgctg ctattctgtc tactgcaaaa
781 agatccatgc ccagtggcag aaagagggaa atgataaaga ttttaccttg aatgattttg
841 gcttcatgat ctttcactca ccatattgta aactggttca gaaatctcta gctcggatgt
901 tgctgaatga cttccttaat gaccagaata gagataaaaa tagtatctat agtggcctgg
961 aagcctttgg ggatgttaaa ttagaagaca cctactttga tagagatgtg gagaaggcat
1021 ttatgaaggc tagctctgaa ctcttcagtc agaaaacaaa ggcatcttta cttgtatcaa
1081 atcaaaatgg aaatatgtac acatcttcag tatatggttc ccttgcatct gttctagcac
1141 agtactcacc tcagcaatta gcagggaaga gaattggagt gttttcttat ggttctggtt
1201 tggctgccac tctgtactct cttaaagtca cacaagatgc tacaccgggg tctgctcttg
1261 ataaaataac agcaagttta tgtgatctta aatcaaggct tgattcaaga actggtgtgg
1321 caccagatgt cttcgctgaa aacatgaagc tcagagagga cacctatcat ttggtcaact
1381 atattcccca gggttcaata gattcactct ttgaaggaac gtggtactta gttagggtgg
1441 atgaaaagca cagaagaact tacgctcggc gtcccactcc aaatgatgac actttggatg
1501 aaggagtagg acttgtgcat tcaaacatag caactgagca tattccaagc cctgccaaga
1561 aagtaccaag actccctgcc acagcagcag aacctgaagc agctgtcatt agtaatgggg
1621 aacattaaga tactctgtga ggtgcaagac ttcagggtgg ggtgggcatg gggtgggggt
1681 atgggaacag ttggaggaat gggatatctg gggataattt taaaggatta catgttatgt
1741 aaatttttat gtgactgaca tggagcctgg atgactatcg tgtacttggg aaagtctctt
1801 tgctctattt gctgacatgc ttcctgttgt ggtctggcca atgccaaatg tactcgaatg
1861 atgttaaggg ctctgtaaaa cttcatacct ctttggccat ttgtatgcat gatgtttggt
1921 ttttaaacat ggtataatga attgtgtact tctgtcagaa gaaagcagag gtactaatct
1981 ccaattaaaa aattttttaa catgtaagaa ttttgtactt tgaacaacaa gattacagaa
2041 agtacctgtg gtttttggaa aacatttcta gcttggggaa tgtgacaaca ttccccagtg
2101 tggtaaaatt ggggtaaaat gtggtaaaat gtgatacgca caaacccttt gaaaatagca
2161 aaacaaacat gccctttttc taaaattgat aaatcctaaa gaggaagaaa agagctggga
2221 caataaaaca ctggctctgg aatctggaat gttaagtcca ggccagcagt gacaaaagtt
2281 attgtaatga cctctgaaca gagaaacact gccattgaag aggcttctgg tatagaaaac
2341 atggtacatt caggagctgt gaatatagct ctaggtgtgc tcctgaatca gttcatggta
2401 gattatgctg aacaacagtg agatgttatt ggaggtgtgg atgagggagt ttgttgttgc
2461 agtccttctt tgcaccttat tttaaagaat aaatgaaaca tttttctggt tactttttta
2521 aaaatttaaa atggaaggga agaatagggg cagggcatta ttaggctatt tctgatgctt
2581 cagtgttata aattcaacat agaggctgac aacctaaatt catggtgtaa cacagctctt
2641 ttccttttcc tttttttttt ttttttttgg tatctgttca atgaaaataa ggtatgaccc
2701 aagtttttac ctagtctgac tagaagtatt ccacttcaag gtctgaagta ggacttttac
2761 cttaaaaaac aacaacaaac aaaactatca cacaggatag ataagaagat tggttaaaca
2821 gttttgtgta gatctttttg gtgctgaact atgacatgag ccttatagat tgtaaaatag
2881 ggatagttgg aactaatgta cagaactaaa ttttttaaac tttatttgct gttaaattct
2941 gtgaagtttc agttatctaa aataaatata cacaaatatg aaatataatg tttcagattg
3001 caaggtaata tgtaatagta gtgtttgtaa gatactcttg tctaatatta actagtagta
3061 ttttgatttg tacagtcata atttgttaaa atgacttcat ttaacattca ctgatgtaga
3121 ttaataatgt aagttctgat ttaaagaatg gtggcaaaat ggtgcatgta atacttttgc
3181 aagtgttggg gagatcggta tgttttgaaa agagtaattt aacttttggg tgccaggaaa
3241 tgggttttct caaagtccat tgccggcaat gggcaggcct gcaaatactg gcacagagca
3301 ttaatcatac accttattaa cggtgaggtg aataactttg aaataaagtt ttagagaaat
3361 gtttcaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3421 a
//