LOCUS BC083512 1570 bp mRNA linear HUM 20-OCT-2004
DEFINITION Homo sapiens thymidylate synthetase, mRNA (cDNA clone MGC:88736
IMAGE:4299083), complete cds.
ACCESSION BC083512
VERSION BC083512.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1570)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1570)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (01-OCT-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 58 Row: b Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4507750.
FEATURES Location/Qualifiers
source 1..1570
/db_xref="H-InvDB:HIT000266042"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:88736 IMAGE:4299083"
/tissue_type="Skin, melanotic melanoma, high MDR."
/clone_lib="NIH_MGC_49"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1570
/gene="TYMS"
/gene_synonym="HsT422"
/gene_synonym="TMS"
/gene_synonym="TS"
/gene_synonym="TSase"
/db_xref="GeneID:7298"
/db_xref="MIM:188350"
CDS 107..1048
/gene="TYMS"
/gene_synonym="HsT422"
/gene_synonym="TMS"
/gene_synonym="TS"
/gene_synonym="TSase"
/codon_start=1
/product="TYMS protein"
/protein_id="AAH83512.1"
/db_xref="GeneID:7298"
/db_xref="MIM:188350"
/translation="MPVAGSELPRRPLPPAAQERDAEPRPPHGELQYLGQIQHILRCG
VRKDDRTGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELS
SKGVKIWDANGSRDFLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQ
LQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGD
MGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPF
PKLRILRKVEKIDDFKAEDFQIEGYNPHPTIKMEMAV"
BASE COUNT 419 a 371 c 402 g 378 t
ORIGIN
1 cggccgcggg aaaaggcgcg cggaaggggt cctgccaccg cgccacttgg cctgcctccg
61 tcccgccgcg ccacttcgcc tgcctccgtc ccccgcccgc cgcgccatgc ctgtggccgg
121 ctcggagctg ccgcgccggc ccttgccccc cgccgcacag gagcgggacg ccgagccgcg
181 tccgccgcac ggggagctgc agtacctggg gcagatccaa cacatcctcc gctgcggcgt
241 caggaaggac gaccgcacgg gcaccggcac cctgtcggta ttcggcatgc aggcgcgcta
301 cagcctgaga gatgaattcc ctctgctgac aaccaaacgt gtgttctgga agggtgtttt
361 ggaggagttg ctgtggttta tcaagggatc cacaaatgct aaagagctgt cttccaaggg
421 agtgaaaatc tgggatgcca atggatcccg agactttttg gacagcctgg gattctccac
481 cagagaagaa ggggacttgg gcccagttta tggcttccag tggaggcatt ttggggcaga
541 atacagagat atggaatcag attattcagg acagggagtt gaccaactgc aaagagtgat
601 tgacaccatc aaaaccaacc ctgacgacag aagaatcatc atgtgcgctt ggaatccaag
661 agatcttcct ctgatggcgc tgcctccatg ccatgccctc tgccagttct atgtggtgaa
721 cagtgagctg tcctgccagc tgtaccagag atcgggagac atgggcctcg gtgtgccttt
781 caacatcgcc agctacgccc tgctcacgta catgattgcg cacatcacgg gcctgaagcc
841 aggtgacttt atacacactt tgggagatgc acatatttac ctgaatcaca tcgagccact
901 gaaaattcag cttcagcgag aacccagacc tttcccaaag ctcaggattc ttcgaaaagt
961 tgagaaaatt gatgacttca aagcggaaga ctttcagatt gaagggtaca atccgcatcc
1021 aactattaaa atggaaatgg ctgtttaggg tgctttcaaa ggagctcgaa ggatattgtc
1081 agtctttagg ggttgggctg gatgccgagg taaaagttct ttttgctcta aaagaaaaag
1141 gaactaggtc aaaaatctgt ccgtgaccta tcagttatta atttttaagg atgttgccac
1201 tggcaaatgt aactgtgcca gttctttcca taataaaagg ctttgagtta actcactgag
1261 ggtatctgac aatgctgagg ttatgaacaa agtgaggaga atgaaatgta tgtgctctta
1321 gcaaaaacat gtatgtgcat ttcaatccca cgtacttata aagaaggttg gtgaatttca
1381 caagctattt ttggaatatt tttagaatat tttaagaatt tcacaagcta ttccctcaaa
1441 tctgagggag ctgagtaaca ccatcgatca tgatgtagag tgtggttatg aactttaaag
1501 ttatagttgt tttatatgtt gctataataa agaagtgttc tgcattcgtc aaaaaaaaaa
1561 aaaaaaaaaa
//