LOCUS BC082263 1480 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens choline kinase beta, mRNA (cDNA clone MGC:99491
IMAGE:6384695), complete cds.
ACCESSION BC082263
VERSION BC082263.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1480)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1480)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-SEP-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP/Gazdar
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 59 Row: l Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 23238259.
FEATURES Location/Qualifiers
source 1..1480
/db_xref="H-InvDB:HIT000265978"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:99491 IMAGE:6384695"
/tissue_type="Lung, large cell carcinoma"
/clone_lib="NIH_MGC_18"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1480
/gene="CHKB"
/gene_synonym="CHETK"
/db_xref="GeneID:1120"
/db_xref="HGNC:HGNC:1938"
CDS 56..1243
/gene="CHKB"
/gene_synonym="CHETK"
/codon_start=1
/product="choline kinase beta"
/protein_id="AAH82263.1"
/db_xref="GeneID:1120"
/db_xref="HGNC:HGNC:1938"
/translation="MAAEATAVAGSGAVGGCLAKDGLQQSKCPDTTPKRRRASSLSRD
AERRAYQWCREYLGGAWRRVQPEELRVYPVSGGLSNLLFRCSLPDHLPSVGEEPREVL
LRLYGAILQGVDSLVLESVMFAILAERSLGPQLYGVFPEGRLEQYIPSRPLKTQELRE
PVLSAAIATKMAQFHGMEMPFTKEPHWLFGTMERYLKQIQDLPPTGLPEMNLLEMYSL
KDEMGNLRKLLESTPSPVVFCHNDIQEGNILLLSEPENADSLMLVDFEYSSYNYRGFD
IGNHFCEWVYDYTHEEWPFYKARPTDYPTQEQQLHFIRHYLAEAKKGETLSQEEQRKL
EEDLLVEVSRYALASHFFWGLWSILQASMSTIEFGYLDYAQSRFQFYFQQKGQLTSVH
SSS"
BASE COUNT 335 a 420 c 431 g 294 t
ORIGIN
1 cccgtccgaa gggagcggag cgcagcctgg cctggggccc ggtcgagccc gcgccatggc
61 ggccgaggcg acagctgtgg ccggaagcgg ggctgttggc ggctgcctgg ccaaagacgg
121 cttgcagcag tctaagtgcc cggacactac cccaaaacgg cggcgcgcct cgtcgctgtc
181 gcgtgacgcc gagcgccgag cctaccaatg gtgccgggag tacttgggcg gggcctggcg
241 ccgagtgcag cccgaggagc tgagggttta ccccgtgagc ggaggcctca gcaacctgct
301 cttccgctgc tcgctcccgg accacctgcc cagcgttggc gaggagcccc gggaggtgct
361 tctgcggctg tacggagcca tcttgcaggg cgtggactcc ctggtgctag aaagcgtgat
421 gttcgccata cttgcggagc ggtcgctggg gccccagctg tacggagtct tcccagaggg
481 ccggctggaa cagtacatcc caagtcggcc attgaaaact caagagcttc gagagccagt
541 gttgtcagca gccattgcca cgaagatggc gcaatttcat ggcatggaga tgcctttcac
601 caaggagccc cactggctgt ttgggaccat ggagcggtac ctaaaacaga tccaggacct
661 gcccccaact ggcctccctg agatgaacct gctggagatg tacagcctga aggatgagat
721 gggcaacctc aggaagttac tagagtctac cccatcgcca gtcgtcttct gccacaatga
781 catccaggaa gggaacatct tgctgctctc agagccagaa aatgctgaca gcctcatgct
841 ggtggacttc gagtacagca gttataacta taggggcttt gacattggga accatttttg
901 tgagtgggtt tatgattata ctcacgagga atggcctttc tacaaagcaa ggcccacaga
961 ctaccccact caagaacagc agttgcattt tattcgtcat tacctggcag aggcaaagaa
1021 aggtgagacc ctctcccaag aggagcagag aaaactggaa gaagatttgc tggtagaagt
1081 cagtcggtat gctctggcat cccatttctt ctggggtctg tggtccatcc tccaggcatc
1141 catgtccacc atagaatttg gttacttgga ctatgcccag tctcggttcc agttctactt
1201 ccagcagaag gggcagctga ccagtgtcca ctcctcatcc tgactccacc ctcccactcc
1261 ttggatttct cctggagcct ccagggcagg accttggagg gaggaacaac gagcagaagg
1321 ccctggcgac tgggctgagc ccccaagtga aactgaggtt caggagaccg gcctgtccct
1381 gagtttgagt aggtccccat ggctggcagg ccagagcccc gtgctgtgta tgtaacacaa
1441 taaacaagct tcttcttcaa aaaaaaaaaa aaaaaaaaaa
//