LOCUS BC080514 1927 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens histidyl-tRNA synthetase, mRNA (cDNA clone MGC:99562
IMAGE:2821065), complete cds.
ACCESSION BC080514
VERSION BC080514.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1927)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1927)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (27-AUG-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 59 Row: a Column: 5.
FEATURES Location/Qualifiers
source 1..1927
/db_xref="H-InvDB:HIT000265786"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:99562 IMAGE:2821065"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1927
/gene="HARS"
/gene_synonym="FLJ20491"
/gene_synonym="HRS"
/db_xref="GeneID:3035"
/db_xref="HGNC:HGNC:4816"
/db_xref="MIM:142810"
CDS 29..1558
/gene="HARS"
/gene_synonym="FLJ20491"
/gene_synonym="HRS"
/codon_start=1
/product="histidyl-tRNA synthetase"
/protein_id="AAH80514.1"
/db_xref="GeneID:3035"
/db_xref="HGNC:HGNC:4816"
/db_xref="MIM:142810"
/translation="MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQ
LGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKET
LMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDN
PAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILD
GMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGG
VSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGV
IYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSI
VEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLN
QLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCI
C"
BASE COUNT 517 a 439 c 562 g 409 t
ORIGIN
1 cagcaggcgg ctcaagtgga cagccgggat ggcagagcgt gcggcgctgg aggagctggt
61 gaaacttcag ggagagcgcg tgcgaggcct caagcagcag aaggccagcg ccgagctgat
121 cgaggaggag gtggcgaaac tcctgaaact gaaggcacag ctgggtcctg atgaaagcaa
181 acagaaattt gtgctcaaaa cccccaaggg cacaagagac tatagtcccc ggcagatggc
241 agttcgcgag aaggtgtttg acgtaatcat ccgttgcttc aagcgccacg gtgcagaagt
301 cattgataca cctgtatttg aactaaagga aacactgatg ggaaagtatg gggaagactc
361 caagcttatc tatgacctga aggaccaggg cggggagctc ctgtcccttc gctatgacct
421 cactgttcct tttgctcggt atttggcaat gaataaactg accaacatta aacgctacca
481 catagcaaag gtatatcggc gggataaccc agccatgacc cgtggccgat accgggaatt
541 ctaccagtgt gattttgaca ttgctgggaa ctttgatccc atgatccctg atgcagagtg
601 cctgaagatc atgtgcgaga tcctgagttc acttcagata ggcgacttcc tggtcaaggt
661 aaacgatcga cgcattctag atgggatgtt tgctatctgt ggtgtttctg acagcaagtt
721 ccgtaccatc tgctcctcag tagacaagct ggacaaggtg tcctgggaag aggtgaagaa
781 tgagatggtg ggagagaagg gccttgcacc tgaggtggct gaccgcattg gggactatgt
841 ccagcaacat ggtggggtat ccctggtgga acagctgctc caggatccta aactatccca
901 aaacaagcag gccttggagg gcctgggaga cctgaagttg ctctttgagt acctgaccct
961 atttggcatt gatgacaaaa tctcctttga cctgagcctt gctcgagggc tggattacta
1021 cactggggtg atctatgagg cagtgctgct acagacccca gcccaggcag gggaagagcc
1081 cctgggtgtg ggcagtgtgg ctgctggagg acgctatgat gggctagtgg gcatgttcga
1141 ccccaaaggg cgcaaggtgc catgtgtggg gctcagcatt ggggtggagc ggattttctc
1201 catcgtggaa cagagactag aggctttgga ggagaagata cggaccacgg agacacaggt
1261 gcttgtggca tctgcacaga agaagctgct agaggaaaga ctaaagcttg tctcagaact
1321 gtgggatgct gggatcaagg ctgagctgct gtacaagaag aacccaaagc tactgaacca
1381 gttacagtac tgtgaggagg caggcatccc actggtggct atcatcggcg agcaggaact
1441 caaggatggg gtcatcaagc tccgttcagt gacgagcagg gaagaggtgg atgtccgaag
1501 agaagacctt gtggaggaaa tcaaaaggag aacaggccag cccctctgca tctgctgaac
1561 tgaacaaact atcagaggaa aggaagtggg actggcacta tttgaggtta agacaaactg
1621 catatgtact tcaattgctt tgcacttttc cgtttcagcg gaagacctga agagtggtca
1681 gaacagagcc tttgattttt attatggtta ttttattgat tattactggc aaaaacggcc
1741 aggtacaaca cctttttcat acaaggccca ggaggcttag tccagtctgt gctcctgggc
1801 tacaaggacc cagcctgaga tggtcccatc tgcagggccc cgcaccagtt ggagcagatg
1861 cctccccacc accaattgcc aaaggtccaa taaaatgcct caaccacgga aaaaaaaaaa
1921 aaaaaaa
//