LOCUS BC080196 1238 bp mRNA linear HUM 17-AUG-2004
DEFINITION Homo sapiens eukaryotic translation initiation factor 5A, mRNA
(cDNA clone MGC:87833 IMAGE:6528111), complete cds.
ACCESSION BC080196
VERSION BC080196.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1238)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1238)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (16-AUG-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 174 Row: p Column: 2
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 20070167.
FEATURES Location/Qualifiers
source 1..1238
/db_xref="H-InvDB:HIT000265785"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:87833 IMAGE:6528111"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1238
/gene="EIF5A"
/gene_synonym="EIF-5A"
/gene_synonym="EIF5A1"
/db_xref="GeneID:1984"
/db_xref="MIM:600187"
CDS 108..572
/gene="EIF5A"
/gene_synonym="EIF-5A"
/gene_synonym="EIF5A1"
/codon_start=1
/product="EIF5A protein"
/protein_id="AAH80196.1"
/db_xref="GeneID:1984"
/db_xref="MIM:600187"
/translation="MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMS
TSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLL
QDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK"
BASE COUNT 280 a 338 c 345 g 275 t
ORIGIN
1 ggcggcggcg gcggcggcag cgggctcgga ggcagcggtt gggctcgcgg cgagcggacg
61 gggtcgagtc agtgcgttcg cgcgagttgg aatcgaagcc tcttaaaatg gcagatgact
121 tggacttcga gacaggagat gcaggggcct cagccacctt cccaatgcag tgctcagcat
181 tacgtaagaa tggctttgtg gtgctcaaag gccggccatg taagatcgtc gagatgtcta
241 cttcgaagac tggcaagcac ggccacgcca aggtccatct ggttggtatt gacatcttta
301 ctgggaagaa atatgaagat atctgcccgt caactcataa tatggatgtc cccaacatca
361 aaaggaatga cttccagctg attggcatcc aggatgggta cctatcactg ctccaggaca
421 gcggggaggt acgagaggac cttcgtctcc ctgagggaga ccttggcaag gagattgagc
481 agaagtacga ctgtggagaa gagatcctga tcacggtgct gtctgccatg acagaggagg
541 cagctgttgc aatcaaggcc atggcaaaat aactggctcc cagggtggcg gtggtggcag
601 cagtgatcct ctgaacctgc agaggccccc tccccgagcc tggcctggct ctggcccggt
661 cctaagctgg actcctccta cacaatttat ttgacgtttt attttggttt tccccacccc
721 ctcaatctgt cggggagccc ctgcccttca cctagctccc ttggccagga gcgagcgaag
781 ctgtggcctt ggtgaagctg ccctcctctt ctcccctcac actacagccc tggtggggga
841 gaagggggtg ggtgctgctt gtggtttagt cttttttttt tttttaattc aatctggaat
901 cagaaagcgg tggattctgg caaatggtcc ttgtgccctc cccactcatc cctggtctgg
961 tcccctgttg cctatagccc tttaccctga gcaccacccc aacagactgg ggaccagccc
1021 cctcgcctgc ctgtgtctct ccccaaaccc ctttagatgg ggagggaaga ggaggagagg
1081 ggaggggacc tgccccctcc tcaggcatct gggagggccc tgcccccatg ggctttaccc
1141 ttccctgcgg gctctctccc cgacacattt gttaaaatca aacctgaata aaactacaag
1201 tttaatatga aaaaaaaaaa aaaaaaaaaa aaaaaaaa
//