LOCUS BC080190 3459 bp mRNA linear HUM 03-JAN-2005
DEFINITION Homo sapiens integrin, alpha 4 (antigen CD49D, alpha 4 subunit of
VLA-4 receptor), mRNA (cDNA clone IMAGE:6069246), complete cds.
ACCESSION BC080190
VERSION BC080190.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3459)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3459)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (16-AUG-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 174 Row: h Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..3459
/db_xref="H-InvDB:HIT000265779_03"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6069246"
/tissue_type="Testis, embryonal carcinoma"
/clone_lib="NIH_MGC_92"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3459
/gene="ITGA4"
/gene_synonym="CD49D"
/gene_synonym="IA4"
/db_xref="GeneID:3676"
/db_xref="MIM:192975"
CDS 374..1207
/gene="ITGA4"
/gene_synonym="CD49D"
/gene_synonym="IA4"
/codon_start=1
/product="ITGA4 protein"
/protein_id="AAH80190.1"
/db_xref="GeneID:3676"
/db_xref="MIM:192975"
/translation="MDNLKHSRVTVAIPLKYEVKLTVHGFVNPTSFVYGSNDENEPET
CMVEKMNLTFHVINTGNSMAPNVSVEIMVPNSFSPQTDKLFNILDVQTTTGECHFENY
QRVCALEQQKSAMQTLKGIVQFLSKTDKRLLYCIKADPHCLNFLCNFGKMESGKEASV
HIQLEGRPSILEMDETSALKFEIRATGFPEPNPRVIELNKDENVAHVLLEGLHHQRPK
RYFTIVIISSSLLLGLIVLLLISYVMWKAGFFKRQYKSILQEENRRDSWSYINSKSND
D"
BASE COUNT 1175 a 544 c 628 g 1112 t
ORIGIN
1 gtttttgaag ccccatgaaa ataaaacata tcttgctgtt gggagtatga agacattgat
61 gttgaatgtg tccttgttta atgctggaga tgatgcatat gaaacgactc tacatgtcaa
121 actacccgtg ggtctttatt tcattaagat tttagagctg ttgtttcatt tttcccatta
181 ggaagagaag caaataaact gtgaagtcac agataactct ggcgtggtac aacttgactg
241 cagtattggc tatatatatg tagatcatct ctcaaggata gatattagct ttctcctgga
301 tgtgagctca ctcagcagag cggaagagga cctcagtatc acagtgcatg ctacctgtga
361 aaatgaagag gaaatggaca atctaaagca cagcagagtg actgtagcaa tacctttaaa
421 atatgaggtt aagctgactg ttcatgggtt tgtaaaccca acttcatttg tgtatggatc
481 aaatgatgaa aatgagcctg aaacgtgcat ggtggagaaa atgaacttaa ctttccatgt
541 tatcaacact ggcaatagta tggctcccaa tgttagtgtg gaaataatgg taccaaattc
601 ttttagcccc caaactgata agctgttcaa cattttggat gtccagacta ctactggaga
661 atgccacttt gaaaattatc aaagagtgtg tgcattagag cagcaaaaga gtgcaatgca
721 gaccttgaaa ggcatagtcc agttcttgtc caagactgat aagaggctat tgtactgcat
781 aaaagctgat ccacattgtt taaatttctt gtgtaatttt gggaaaatgg aaagtggaaa
841 agaagccagt gttcatatcc aactggaagg ccggccatcc attttagaaa tggatgagac
901 ttcagcactc aagtttgaaa taagagcaac aggttttcca gagccaaatc caagagtaat
961 tgaactaaac aaggatgaga atgttgcgca tgttctactg gaaggactac atcatcaaag
1021 acccaaacgt tatttcacca tagtgattat ttcaagtagc ttgctacttg gacttattgt
1081 acttctgttg atctcatatg ttatgtggaa ggctggcttc tttaaaagac aatacaaatc
1141 tatcctacaa gaagaaaaca gaagagacag ttggagttat atcaacagta aaagcaatga
1201 tgattaagga cttctttcaa attgagagaa tggaaaacag actcaggttg tagtaaagaa
1261 atttaaaaga cactgtttac aagaaaaaat gaattttgtt tggacttctt ttactcatga
1321 tcttgtgaca tattatgtct tcatgcaagg ggaaaatctc agcaatgatt actctttgag
1381 atagaagaac tgcaaaggta ataatacagc caaagataat ctctcagctt ttaaatgggt
1441 agagaaacac taaagcattc aatttattca agaaaagtaa gcccttgaag atatcttgaa
1501 atgaaagtat aactgagtta aattatactg gagaagtctt agacttgaaa tactacttac
1561 catatgtgct tgcctcagta aaatgaaccc cactgggtgg gcagaggttc atttcaaata
1621 catctttgat acttgttcaa aatatgttct ttaaaaatat aattttttag agagctgttc
1681 ccaaattttc taacgagtgg accattatca ctttaaagcc ctttatttat aatacatttc
1741 ctacgggctg tgttccaaca accatttttt ttcagcagac tatgaatatt atagtattat
1801 aggccaaact ggcaaacttc agactgaaca tgtacactgg tttgagctta gtgaaatgac
1861 ttctggataa ttattttttt ataattatgg atttcaccat ctttctttct gtatatatac
1921 atgtgttttt atgtaggtat atatttacca ttcttcctat ctattcttcc tataacacac
1981 ctttatcaag catacccagg agtaatcttc aaatcttttg ttatattctg aaacaaaaga
2041 ttgtgagtgt tgcactttac ctgatacatg ctgatttaga aaatacagaa accatacctc
2101 actaataact ttaaaatcaa agctgtgcaa agactagggg gcctatactt catatgtatt
2161 atgtactatg taaaatattg actatcacac aactatttcc ttggatgtaa ttctttgtta
2221 ccctttacaa gtataagtgt taccttacat ggaaacgaag aaacaaaatt cataaattta
2281 aattcataaa tttagctgaa agatactgat tcaatttgta tacagtgaat ataaatgaga
2341 cgacagcaaa attttcatga aatgtaaaat atttttatag tttgttcata ctatatgagg
2401 ttctatttta aatgactttc tggattttaa aaaatttctt taaatacaat catttttgta
2461 atatttattt tatgcttatg atctagataa ttgcagaata tcattttatc tgactctgtc
2521 ttcataagag agctgtggcc gaattttgaa catctgttat agggagtgat caaattagaa
2581 ggcaatgtgg aaaaacaatt ctgggaaaga tttctttata tgaagtccct gccactagcc
2641 agccatccta attgatgaaa gttatctgtt cacaggcctg cagtgatggt gaggaatgtt
2701 ctgagatttg cgaaggcatt tgagtagtga aatgtaagca caaaacctcc tgaacccaga
2761 gtgtgtatac acaggaataa actttatgac atttatgtat ttttaaaaaa ctttgtatcg
2821 ttataaaaag gctagtcatt ctttcaggag aacatctagg atcatagatg aaaaatcaag
2881 ccccgattta gaactgtctt ctccaggatt gtctctaagg aaatttacat ttggttcttt
2941 cctactcaga actactcaga aacaactata tatttcaggt tatttgagca cagtgaaagc
3001 agagtactat ggttgtccaa cacaggcctc tcagatacaa ggggaacaca attacatatt
3061 gggctagatt ttgcccagtt caaaatagta tttgttatca acttactttg ttacttgtat
3121 catgaatttt aaaaccctac cactttaaga agacagggat gggttattct tttttggcag
3181 gtaggctata taactatgtg attttgaaat ttaactgctc tggattaggg agcagtgaat
3241 caaggcagac ttatgaaatc tgtattatat ttgtaacaga atataggaaa tttaacataa
3301 ttgatgagct caaatcctga aaaatgaaag aatccaaatt atttcagaat tatctaggtt
3361 aaatattgat gtattatgat ggttgcaaag ttttttgtgt gtccaataaa cacattgtaa
3421 aaaaaagaat ttgaattgat atttaaaaaa aaaaaaaaa
//