LOCUS BC078663 5048 bp mRNA linear HUM 04-AUG-2008
DEFINITION Homo sapiens amylo-1, 6-glucosidase, 4-alpha-glucanotransferase,
mRNA (cDNA clone MGC:88304 IMAGE:30350685), complete cds.
ACCESSION BC078663
VERSION BC078663.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5048)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 5048)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-AUG-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. Michael Brownstein and Dr. Miklos Palkovits
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 57 Row: o Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4557284.
FEATURES Location/Qualifiers
source 1..5048
/db_xref="H-InvDB:HIT000265750"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:88304 IMAGE:30350685"
/tissue_type="Glandular pool- thyroid, parathyroid,
adrenal cortex, pineal gland, submandibular gland."
/clone_lib="NIH_MGC_184"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..5048
/gene="AGL"
/gene_synonym="GDE"
/db_xref="GeneID:178"
/db_xref="HGNC:HGNC:321"
/db_xref="MIM:610860"
CDS 121..4719
/gene="AGL"
/gene_synonym="GDE"
/codon_start=1
/product="amylo-1, 6-glucosidase,
4-alpha-glucanotransferase"
/protein_id="AAH78663.1"
/db_xref="GeneID:178"
/db_xref="HGNC:HGNC:321"
/db_xref="MIM:610860"
/translation="MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAV
TVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKS
GGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHF
TPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVY
NHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIE
NDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLT
IIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYH
QEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPN
KACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAH
MKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNV
FVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDI
THDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNP
EALPSNTGEVNFQSGIIAARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIH
QSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSING
TPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHA
QVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASLASRLTLAELNQILYRCESE
EKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSN
RLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFV
QNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGL
PHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYA
RYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVI
QEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDK
MGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAI
KVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLR
PNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDN
YNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLER
SPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL"
BASE COUNT 1585 a 908 c 1076 g 1479 t
ORIGIN
1 agagtcgcgg gtcctgcagt gccccagaag ccgcacgtat aactccctcg gcgggtaact
61 cattcgactg tggagttctt ttaattctta tgaaagattt caaatcctct agaagccaaa
121 atgggacaca gtaaacagat tcgaatttta cttctgaacg aaatggagaa actggaaaag
181 accctcttca gacttgaaca agggtatgag ctacagttcc gattaggccc aactttacag
241 ggaaaagcag ttaccgtgta tacaaattac ccatttcctg gagaaacatt taatagagaa
301 aaattccgtt ctctggattg ggaaaatcca acagaaagag aagatgattc tgataaatac
361 tgtaaactta atctgcaaca atctggttca tttcagtatt atttccttca aggaaatgag
421 aaaagtggtg gaggttacat agttgtggac cccattttac gtgttggtgc tgataatcat
481 gtgctaccct tggactgtgt tactcttcag acatttttag ctaagtgttt gggacctttt
541 gatgaatggg aaagcagact tagggttgca aaagaatcag gctacaacat gattcatttt
601 accccattgc agactcttgg actatctagg tcatgctact cccttgccaa tcagttagaa
661 ttaaatcctg acttttcaag acctaataga aagtatacct ggaatgatgt tggacagcta
721 gtggaaaaat taaaaaagga atggaatgtt atttgtatta ctgatgttgt ctacaatcat
781 actgctgcta atagtaaatg gatccaggaa catccagaat gtgcctataa tcttgtgaat
841 tctccacact taaaacctgc ctgggtctta gacagagcac tttggcgttt ctcctgtgat
901 gttgcagaag ggaaatacaa agaaaaggga atacctgctt tgattgaaaa tgatcaccat
961 atgaattcca tccgaaaaat aatttgggag gatatttttc caaagcttaa actctgggaa
1021 tttttccaag tagatgtcaa caaagcggtt gagcaattta gaagacttct tacacaagaa
1081 aataggcgag taaccaagtc tgatccaaac caacacctta cgattattca agatcctgaa
1141 tacagacggt ttggctgtac tgtagatatg aacattgcac taacgacttt cataccacat
1201 gacaaggggc cagcagcaat tgaagaatgc tgtaattggt ttcataaaag aatggaggaa
1261 ttaaattcag agaagcatcg actcattaac tatcatcagg aacaggcagt taattgcctt
1321 ttgggaaatg tgttttatga acgactggct ggccatggtc caaaactagg acctgtcact
1381 agaaagcatc ctttagttac caggtatttt actttcccat ttgaagagat agacttctcc
1441 atggaagaat ctatgattca tctgccaaat aaagcttgtt ttctgatggc acacaatgga
1501 tgggtaatgg gagatgatcc tcttcgaaac tttgctgaac cgggttcaga agtttaccta
1561 aggagagaac ttatttgctg gggagacagt gttaaattac gctatgggaa taaaccagag
1621 gactgtcctt atctctgggc acacatgaaa aaatacactg aaataactgc aacttatttc
1681 cagggagtac gtcttgataa ctgccactca acacctcttc acgtagctga gtacatgttg
1741 gatgctgcta ggaatttgca acccaattta tatgtagtag ctgaactgtt cacaggaagt
1801 gaagatctgg acaatgtctt tgttactaga ctgggcatta gttccttaat aagagaggca
1861 atgagtgcat ataatagtca tgaagagggc agattagttt accgatatgg aggagaacct
1921 gttggatcct ttgttcagcc ctgtttgagg cctttaatgc cagctattgc acatgccctg
1981 tttatggata ttacgcatga taatgagtgt cctattgtgc atagatcagc gtatgatgct
2041 cttccaagta ctacaattgt ttctatggca tgttgtgcta gtggaagtac aagaggctat
2101 gatgaattag tgcctcatca gatttcagtg gtttctgaag aacggtttta cactaagtgg
2161 aatcctgaag cattgccttc aaacacaggt gaagttaatt tccaaagcgg cattattgca
2221 gccaggtgtg ctatcagtaa acttcatcag gagcttggag ccaagggttt tattcaggtg
2281 tatgtggatc aagttgatga agacatagtg gcagtaacaa gacactcacc tagcatccat
2341 cagtctgttg tggctgtatc tagaactgct ttcaggaatc ccaagacttc attttacagc
2401 aaggaagtgc ctcaaatgtg catccctggc aaaattgaag aagtagttct tgaagctaga
2461 actattgaga gaaacacgaa accttatagg aaggatgaga attcaatcaa tggaacacca
2521 gatatcacag tagaaattag agaacatatt cagcttaatg aaagtaaaat tgttaaacaa
2581 gctggagttg ccacaaaagg gcccaatgaa tatattcaag aaatagaatt tgaaaacttg
2641 tctccaggaa gtgttattat attcagagtt agtcttgatc cacatgcaca agtcgctgtt
2701 ggaattcttc gaaatcatct gacacaattc agtcctcact ttaaatctgg cagcctagct
2761 gttgacaatg cagatcctat attaaaaatt ccttttgctt ctcttgcctc cagattaact
2821 ttggctgagc taaatcagat cctttaccga tgtgaatcag aagaaaagga agatggtgga
2881 gggtgctatg acataccaaa ctggtcagcc cttaaatatg caggtcttca aggtttaatg
2941 tctgtattgg cagaaataag accaaagaat gacttggggc atcctttttg taataatttg
3001 agatctggag attggatgat tgactatgtc agtaaccggc ttatttcacg atcaggaact
3061 attgctgaag ttggtaaatg gttgcaggct atgttcttct acctgaagca gatcccacgt
3121 taccttatcc catgttactt tgatgctata ttaattggtg catataccac tcttctggat
3181 acagcatgga agcagatgtc aagctttgtt cagaatggtt caacctttgt gaaacacctt
3241 tcattgggtt cagttcaact gtgtggagta ggaaaattcc cttccctgcc aattctttca
3301 cctgccctaa tggatgtacc ttataggtta aatgagatca caaaagaaaa ggagcaatgt
3361 tgtgtttctc tagctgcagg cttacctcat ttttcttctg gtattttccg ctgctgggga
3421 agggatactt ttattgcact tagaggtata ctgctgatta ctggacgcta tgtagaagcc
3481 aggaatatta ttttagcatt tgcgggtacc ctgaggcatg gtctcattcc taatctactg
3541 ggtgaaggaa tttatgccag atacaattgt cgggatgctg tgtggtggtg gctgcagtgt
3601 atccaggatt actgtaaaat ggttccaaat ggtctagaca ttctcaagtg cccagtttcc
3661 agaatgtatc ctacagatga ttctgctcct ttgcctgctg gcacactgga tcagccattg
3721 tttgaagtca tacaggaagc aatgcaaaaa cacatgcagg gcatacagtt ccgagaaagg
3781 aatgctggtc cccagataga tcgaaacatg aaggacgaag gttttaatat aactgcagga
3841 gttgatgaag aaacaggatt tgtttatgga ggaaatcgtt tcaattgtgg cacatggatg
3901 gataaaatgg gagaaagtga cagagctaga aacagaggaa tcccagccac accaagagat
3961 gggtctgctg tggaaattgt gggcctgagt aaatctgctg ttcgctggtt gctggaatta
4021 tccaaaaaaa atattttccc ttatcatgaa gtcacagtaa aaagacatgg aaaggctata
4081 aaggtctcat atgatgagtg gaacagaaaa atacaagaca actttgaaaa gctatttcat
4141 gtttccgaag acccttcaga tttaaatgaa aagcatccaa atctggttca caaacgtggc
4201 atatacaaag atagttatgg agcttcaagt ccttggtgtg actatcagct caggcctaat
4261 tttaccatag caatggttgt ggcccctgag ctctttacta cagaaaaagc atggaaagct
4321 ttggagattg cagaaaaaaa attgcttggt ccccttggca tgaaaacttt agatccagat
4381 gatatggttt actgtggaat ttatgacaat gcattagaca atgacaacta caatcttgct
4441 aaaggtttca attatcacca aggacctgag tggctgtggc ctattgggta ttttcttcgt
4501 gcaaaattat atttttccag attgatgggc ccggagacta ctgcaaagac tatagttttg
4561 gttaaaaatg ttctttcccg acattatgtt catcttgaga gatccccttg gaaaggactt
4621 ccagaactga ccaatgagaa tgcccagtac tgtcctttca gctgtgaaac acaagcctgg
4681 tcaattgcta ctattcttga gacactttat gatttatagt ttattacaga tattaagtat
4741 gcaattactt gtattatagg atgcaaggtc atcatatgta aatgccttat atgcacaggc
4801 tcaagttgtt ttaaaaatct catttattat aatattgatg ctcaattagg taagattgta
4861 aaagcattga ttttttttaa tgtacagagg tagatttcaa tttgaatcag aaagaaatat
4921 cattaccaat gaaatgtgtt tgagttcagt aagaattatt caaatgccta gaaatccata
4981 gtttggaaaa gaaaaatcat gtcatcttct atttgtacag aaaaaaaaaa aaaaaaaaaa
5041 aaaaaaaa
//