LOCUS BC078655 3872 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens Src homology 3 domain-containing guanine nucleotide
exchange factor, mRNA (cDNA clone MGC:87070 IMAGE:4374088),
complete cds.
ACCESSION BC078655
VERSION BC078655.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3872)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3872)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-AUG-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 167 Row: a Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361486.
FEATURES Location/Qualifiers
source 1..3872
/db_xref="H-InvDB:HIT000265742"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:87070 IMAGE:4374088"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3872
/gene="SGEF"
/gene_synonym="CSGEF"
/gene_synonym="DKFZP434D146"
/gene_synonym="HMFN1864"
/db_xref="GeneID:26084"
CDS 153..2768
/gene="SGEF"
/gene_synonym="CSGEF"
/gene_synonym="DKFZP434D146"
/gene_synonym="HMFN1864"
/codon_start=1
/product="Src homology 3 domain-containing guanine
nucleotide exchange factor"
/protein_id="AAH78655.1"
/db_xref="GeneID:26084"
/translation="MDGESEVDFSSNSITPLWRRRSIPQPHQLLGRSKPRPQSYQSPN
GLLITDFPVEDGGTLSAAQIPAQVPTASDSRTVHRSPLLLGAQRRAVANGGTASPEYR
AASPRLRRPKSPKLPKAVPGGSPKSPANGAVTLPAPPPPPVLRPPRTPNAPAPCTPEE
DLTGLTASPVPSPTANGLAANNDSPGSGSQSGRKAKDPERGLFPGPQKSSSEQKLPLQ
RLPSQENELLENPSVVLSTNSPAALKVGKQQIIPKSLASEIKISKSNNQNVEPHKRLL
KVRSMVEGLGGPLGHAGEESEVDNDVDSPGSLRRGLRSTSYRRAVVSGFDFDSPTSSK
KKNRMSQPVLKVVMEDKEKFSSLGRIKKKMLKGQGTFDGEENAVLYQNYKEKALDIDS
DEESEPKEQKSDEKIVIHHKPLRSTWSQLSAVKRKGLSQTVSQEERKRQEAIFEVISS
EHSYLLSLEILIRMFKNSKELSDTMTKTERHHLFSNITDVCEASKKFFIELEARHQNN
IFIDDISDIVEKHTASTFDPYVKYCTNEVYQQRTLQKLLATNPSFKEVLSRIESHEDC
RNLPMISFLILPMQRVTRLPLLMDTICQKTPKDSPKYEVCKRALKEVSKLVRLCNEGA
RKMERTEMMYTINSQLEFKIKPFPLVSSSRWLVKRGELTAYVEDTVLFSRRTSKQQVY
FFLFNDVLIITKKKSEESYNVNDYTLRDQLLVESCDNEELNSSPGKNSSTMLYSRQSS
ASHLFTLTVLSNHANEKVEMLLGAETQSERARWITALGHSSGKPPADRTSLTQVEIVR
SFTAKQPDELSLQVADVVLIYQRVSDGWYEGERLRDGERGWFPMECAKEITCQATIDK
NVERMGRLLGLETNV"
BASE COUNT 1060 a 952 c 929 g 931 t
ORIGIN
1 ggcgctctcc taagctctcg cggctgcgct tcggtcccgg acccgggcca cccacggggt
61 agtgggtgct cctcggcccc ggacattgca agccccagaa ggcaagacta actcggtgtt
121 gctcctcccg gcgctgactt cgaggcccgg ctatggacgg cgagagcgag gtggattttt
181 ctagcaacag cataacccct ttgtggcgga ggcggtcgat tcctcagccc caccagcttc
241 tgggccggag caagccgagg ccccagtcct accagagccc caacgggtta ctaattacgg
301 atttcccggt ggaggacgga gggacgctct ccgcagcgca gattcccgcc caggtgccca
361 ccgcctcgga cagcaggacg gtacatagga gccccctgct tctgggcgcc cagcggagag
421 cggtggccaa tggtgggacg gcatccccgg agtacagggc tgcctctcct cgacttcgac
481 ggcccaagtc acccaagctc cccaaagcgg tgcctggcgg ctccccgaaa tccccagcaa
541 atggcgcggt gaccttgcct gcgccgccgc cgccgccggt tctgcgcccc ccgcggactc
601 ctaacgcgcc cgccccctgc acccccgagg aggaccttac tgggttgact gccagcccgg
661 tgccttcgcc cactgcaaat ggccttgccg ctaataacga ctctcctggg tcaggttcgc
721 agtccggccg gaaggcaaag gaccccgaac gggggctctt tcctgggccc cagaaaagtt
781 cttcggaaca aaaactcccc ctccaaaggc tgccctccca ggagaacgag ctcctcgaga
841 atccttccgt ggttttgagt acaaacagcc ccgccgccct caaagtgggg aagcagcaga
901 tcattccgaa gagtctggcc tcggaaatta aaataagtaa atccaacaat caaaatgtgg
961 agccccacaa gagactcctc aaggtgcgca gcatggtgga gggcctagga ggacccctgg
1021 gtcacgcagg ggaggagagt gaggtcgata acgacgtgga tagcccaggg tctctgcgga
1081 gaggcttgcg gtccacgtct tatcgcaggg cagtggtcag tggctttgat tttgacagtc
1141 ctaccagctc gaagaagaag aacagaatgt cccagcctgt tctgaaagtg gtgatggaag
1201 acaaggagaa gttttccagt ctgggaagga taaagaaaaa aatgctgaaa ggacaaggaa
1261 catttgatgg ggaagaaaat gctgtcctgt atcaaaacta caaggaaaag gcccttgaca
1321 ttgattctga tgaagagtca gagcccaaag aacagaagtc agatgaaaaa attgtgattc
1381 accataagcc attgagatcc acatggagcc aactctctgc ggtgaaaaga aagggattat
1441 ctcagacagt aagccaggag gaaagaaaga gacaagaggc tatctttgaa gtcatatcct
1501 ctgaacattc atatttactc agcttggaga tcttgatacg aatgtttaaa aattctaaag
1561 aactgagtga tacaatgact aaaaccgaga ggcaccatct tttctccaat attacagatg
1621 tctgtgaggc aagcaaaaag ttctttatag agttggaagc aagacatcag aataatatct
1681 tcatagatga cataagtgac attgtggaaa aacacacagc atccacattt gacccatatg
1741 tgaaatactg cacaaatgaa gtctaccaac aacgaacact acaaaaattg ttagctacca
1801 atccatcctt taaggaagta ttgtcaagga ttgagtccca tgaagactgt aggaacttac
1861 ccatgatctc ttttctcatt ctccccatgc agagggtgac ccgccttccc ctgctgatgg
1921 atactatctg tcaaaaaaca cctaaggact ctccgaagta tgaagtctgc aaaagagcct
1981 tgaaggaagt tagcaagttg gttcgactat gcaatgaggg cgcccggaag atggaaagga
2041 ctgagatgat gtacacaatt aactcccagc tggaatttaa aattaagcct tttcctttag
2101 tctcctcttc ccggtggttg gtaaaaagag gtgaattgac agcctatgtt gaagacactg
2161 tgcttttctc aagaaggaca tccaaacagc aagtctactt ctttctcttt aacgatgtgc
2221 tcattatcac caagaagaag agtgaagaaa gttacaacgt caatgattat accttaagag
2281 atcagctatt ggtggaatct tgtgacaatg aagagcttaa ttcttctcca gggaagaaca
2341 gctccacaat gctctattca agacagagct ctgccagtca cctctttact ctgacagtcc
2401 ttagtaacca cgcgaatgag aaagtggaga tgctactagg agctgagacg cagagcgagc
2461 gagcccgctg gataactgcc ctgggacaca gcagcgggaa gccgcctgca gaccgaacct
2521 cactgaccca ggtggaaatc gttaggtcat ttactgctaa gcagccagat gaactctccc
2581 tgcaggtggc tgacgtcgtc ctcatctatc aacgtgtcag cgatggctgg tatgaggggg
2641 aacgactacg agatggagaa agaggctggt ttcctatgga atgtgccaag gagataacat
2701 gtcaagctac aattgataag aatgtggaga gaatgggacg cttgctagga ctggagacca
2761 acgtgtagtc tctcagatgg tcttttgtta ctgcaagatt tgcacgacac ttaccgggct
2821 ggttggttct gggctagttt tattgttaat tttgtcacag cctatttaat taaaagaacg
2881 aaaacacttg cctttaagct tgccaggttg ttctgctctc tcatgagaag agcttggata
2941 cagtgagttt gcacagctca gtttttacct aaccacacac ttgcagacct cctgaggtac
3001 acagaatagc tgagcagttc acttcaggga tcaggtcatc tctgctcctc ctagtttcac
3061 catgttctgg caataaaaaa cacatattat atcctggttt tctctatcct tgcattacta
3121 aggtgactgt ctctctttat acatccttgt atggttctcc cagtattagc aagattgtat
3181 atctgtaaag aatgtccagt tttgtaaata tttccctgcc tttttttttc tttttttaca
3241 tctgatttta atgcttcgtt aacttcaaaa ggaactggta gagttcagaa ggtgagctgt
3301 tgtttttcta aacctcttcc caggaagggg acattgacac ttgaattttt gtcacctttt
3361 tcctcattag aaggaaagta gaaagcctta ctgtaggatt tttaaaaaaa aatccatctc
3421 accccatatt ggtcttaaat aagtatagac taattaacct aagctacctt taacaacgta
3481 gaatttagat gggttcatat atgtgagaaa aacctgaata taggacaggg gtcctacttt
3541 tttccccacc tctgtcgccc aggctagagt atagtggtgt gatcttggcc cactgcaacc
3601 tctgcttcct aggttcaagt gattctcctg cctcagcctc ccaagtagct gggattgtaa
3661 gagtatgcca ccacgcccag ctactttttg tatttttagt agagacaggg tttcatcatg
3721 ttggccagga tggtctctta actcctgccc tcaagtgatc caccagagag gagatcctcg
3781 gcctccccaa gtgctgggat tataggcatg agccaccgtg cccagcctac tttctaatta
3841 attaaaaaaa aaaaaagaaa aaaaaaaaaa aa
//