LOCUS BC078179 3556 bp mRNA linear HUM 01-SEP-2006
DEFINITION Homo sapiens eukaryotic translation initiation factor 2 alpha
kinase 4, mRNA (cDNA clone IMAGE:6503617), complete cds.
ACCESSION BC078179
VERSION BC078179.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3556)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3556)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (19-JUL-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 174 Row: p Column: 14
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..3556
/db_xref="H-InvDB:HIT000265738"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6503617"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3556
/gene="EIF2AK4"
/gene_synonym="GCN2"
/gene_synonym="KIAA1338"
/db_xref="GeneID:440275"
/db_xref="HGNC:HGNC:19687"
/db_xref="MIM:609280"
CDS 51..1901
/gene="EIF2AK4"
/gene_synonym="GCN2"
/gene_synonym="KIAA1338"
/codon_start=1
/product="EIF2AK4 protein"
/protein_id="AAH78179.1"
/db_xref="GeneID:440275"
/db_xref="HGNC:HGNC:19687"
/db_xref="MIM:609280"
/translation="MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPD
ACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNES
VNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQR
LLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPG
GHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPD
QLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKID
KCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHLS
HSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL
ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDL
PADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIP
SNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVWYRVIPSPL"
BASE COUNT 1026 a 750 c 807 g 973 t
ORIGIN
1 ggcccaccgc cgcccaggca aggccgccct gccttgggcg cagcgctgcc atggctgggg
61 gccgtggggc ccccgggcgc ggccgggacg agcctccgga gagctacccg caacgacagg
121 accacgagct acaggccctg gaggccatct acggcgcgga cttccaagac ctgcggccgg
181 acgcttgcgg accggtcaaa gagccccctg aaatcaattt agttttgtac cctcaaggcc
241 taactggtga agaagtatat gtaaaagtgg atttgagggt taaatgccca cctacctatc
301 cagatgtagt tcctgaaata gagttaaaaa atgccaaagg tctatcaaat gaaagtgtca
361 atttgttaaa atctcgccta gaagaactgg ccaagaaaca ctgtggggag gtgatgatct
421 ttgaactggc ttaccacgtg cagtcatttc tcagcgagca taacaagccc cctcccaagt
481 cttttcatga agaaatgctg gaaaggcggg ctcaggagga gcagcagagg ctgttggagg
541 ccaagcggaa agaagagcag gagcaacgtg aaatcctgca tgagattcag agaaggaaag
601 aagagataaa agaagagaaa aaaaggaaag aaatggctaa gcaggaacgt ttggaaattg
661 ctagtttgtc aaaccaagat catacctcta agaaggaccc aggaggacac agaacggctg
721 ccattctaca tggaggctct cctgactttg taggaaatgg taaacatcgg gcaaactcct
781 caggaaggtc taggcgagaa cgtcagtatt ctgtatgtaa tagtgaagat tctcctggct
841 cttgtgaaat tctgtatttc aatatgggga gtcctgatca gctcatggtg cacaaaggga
901 aatgtattgg cagtgatgaa caacttggaa aattagtcta caatgctttg gaaacagcca
961 ctggtggctt tgtcttgttg tatgagtggg tccttcagtg gcagaaaaaa atgggtccat
1021 tccttaccag tcaagaaaaa gagaagattg ataagtgcaa aaagcagatt caaggaacag
1081 aaacagaatt caactcactg gtaaaattga gccatccaaa tgtagtacgc taccttgcaa
1141 tgaatctcaa agagcaagac gactccatcg tggtggacat tttagtggag cacattagtg
1201 gggtctctct tgctgcacac ctgagccact caggccccat ccctgtgcat cagcttcgca
1261 ggtacacagc tcagctcctg tcaggccttg attatctgca cagcaattct gtggtgcata
1321 aggtcctgag tgcatctaat gtcttggtgg atgcagaagg caccgtcaag attacggact
1381 atagcatttc taagcgcctc gcagacattt gcaaggagga tgtgtttgag caaacccgag
1441 ttcgttttag tgacaatgct ctgccttata aaacggggaa gaaaggagat gtttggcgtc
1501 ttggccttct gctgctgtcc ctcagccaag gacaggaatg tggagagtac cctgtgacca
1561 tccctagtga cttaccagct gactttcaag attttctaaa gaaatgtgtg tgcttggatg
1621 acaaggaaag atggagtccc cagcagttgt tgaaacacag ctttataaat ccccagccaa
1681 aaatgcctct agtggaacaa agtcctgaag attctgaagg acaagattat gttgagactg
1741 ttattcctag caaccggcta cccagtgctg ccttctttag tgagacacag agacagtttt
1801 cccgatactt cattgagttt gaagaattac aacttcttgg taaaggagct tttggagctg
1861 tcatcaaggt gtggtacaga gtcattccca gtccccttta gagctccttc tctgttccat
1921 ttccacaaag actcatggac tttactttta ttttgggagt ggggagggaa gtgaatatgg
1981 ctgttggata ctatgctcat cctcaccttc ctggagttaa aggttttatt taagttacgc
2041 tggacacagt tgagaattgt cccactcctc tttccttgct tcatgtggag cattaacatg
2101 aaacacacat ggttataaca gcacttcaag tggaatcaag acctccacag aaagaacatt
2161 tttgtgtgct ttggttcaga aagaacattg ttcttttttt ctcctgtttg ctgttagagt
2221 tgctgtagat aaaaaggttc taaggttaga agagagatgg acttcaaact gggagatggc
2281 cccagcaggt ctcgatggct tttattttcc agctattatt gggacaggaa agtagcgatg
2341 tattattaag gtacactgtt gttgggtctt cctttcttcg ttctagtcta tttgggtgac
2401 ttttattatt atctgcccct tgtagaaaga ggtttcagct gtttttatat aatagaagta
2461 tttctttgta aattaggaga cccagattat tgtattaagt ccatatacag tacatgtcat
2521 ctttcacata tgaaggtaac ccaccatcaa ctgagtatat tgtctgttaa ggattctgag
2581 agtatcatta ggaagcatta ctgttactgt agaaaaactt ttacactaaa aaaggaatta
2641 aattagtttt cctgtggtaa aagacagaca agcaagacag atgtcctgat cagaaagatc
2701 ataattatgt ctaagaggag gggatgattg aagcgtatac tgtcccttta acacaatatc
2761 attttgatca aatcctttaa cttcattcta ttttaaattc ttgaacgtag aatagtaaca
2821 aatttcatct ctttacaagt acttaataat gataggcaag ctcatataac aaaaacatct
2881 gagtaactta agcccccacg gtttccagta gtttctccct gtgagaatcc acagccctca
2941 cctcctacct ctgtgcttct gtgtcagagt agggcagcct gcccagtgat ctggcattca
3001 ttagcagttg gacccattac tgtttctgca ccttttcact tcagtaaatg aattcctagg
3061 ggtttccagt atagcaagct ttactcccaa cccagttcgt atatgatgtg tactgagaca
3121 ggagatcgaa aggcaatgcc aagatcccca cctcccgccc tacactgtcc tcttccctat
3181 taatgtggca gtaaaagaac acagggctat cattttttgg agcacctgcc atatttcaga
3241 ttgtgtgcca ggtgatttac aaaccttgtc tttaaatctc aggacattct tacgagatgg
3301 gtatgatcct cattccctag atgagggaac tgaagctcag agaaggttat gtcctttgcc
3361 tgagataagg gagataaaac cagcattcag acccacctct gttgtactac aaagtgtgtg
3421 ttcttgtcac tatttccata caaaaaaaga taatctgaaa ctcaattttg tagtacttcc
3481 ttcttattcc caggacttca cttagacttt tattagttta attgaaactt gtgagccaaa
3541 caaaaaaaaa aaaaaa
//