LOCUS BC078147 4199 bp mRNA linear HUM 27-JUL-2004
DEFINITION Homo sapiens DiGeorge syndrome critical region gene 8, mRNA (cDNA
clone MGC:87832 IMAGE:6014346), complete cds.
ACCESSION BC078147
VERSION BC078147.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4199)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4199)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (19-JUL-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 174 Row: f Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 20070318.
FEATURES Location/Qualifiers
source 1..4199
/db_xref="H-InvDB:HIT000265706"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:87832 IMAGE:6014346"
/tissue_type="Testis, embryonal carcinoma"
/clone_lib="NIH_MGC_92"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..4199
/gene="DGCR8"
/gene_synonym="FLJ21774"
/gene_synonym="FLJ21886"
/gene_synonym="FLJ22127"
/gene_synonym="Gy1"
/db_xref="GeneID:54487"
CDS 144..2366
/gene="DGCR8"
/gene_synonym="FLJ21774"
/gene_synonym="FLJ21886"
/gene_synonym="FLJ22127"
/gene_synonym="Gy1"
/codon_start=1
/product="DGCR8 protein"
/protein_id="AAH78147.1"
/db_xref="GeneID:54487"
/translation="METDESPSPLPCGPAGEAVMESRARPFQALPREQSPPPPLQTSS
GAEVMDVGSGGDGQSELPAEDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHA
PAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGAERDVRAECGLLLSPVSGDVH
ACPFGGSVGDGVGIGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGAGG
FTAKAIVQRDRVDEEALNFPYEDDFDNDVDALLEEGLCAPKKRRTEEKYGGDSDHPSD
GETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYF
LGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQSSDLTPSGDVSPVKPLSRSAELEFPL
DEPDSMGADPGPPDEKDPLGAEAAPGALGQVKAKVEVCKDESVDLEEFRSYLEKRFDF
EQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPN
GKSEVCILHEYMQRVLKVRPVYNFFECARATLEILIPDFVKQTSEEKPKDSEELEYFN
HISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMAC
GKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSV
IELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV
"
BASE COUNT 1031 a 1058 c 1188 g 922 t
ORIGIN
1 ggtggcaagc ttgactaagc cgccagcgca cagcgcggca ggacgcgccc gggtctcagc
61 ggacttgtgc atgttagctg tgtagattta tgtgagggct tgtaaaactc tggtcttgta
121 aactagtctt aagcgctttt aatatggaga cagatgagag cccctctccg ctcccgtgtg
181 ggcccgcagg agaagcggtg atggagagcc gagctcgccc cttccaagcg ctgccccgtg
241 agcagtctcc accacctccc ctgcaaacgt ccagtggtgc agaggtaatg gacgttggct
301 ctggtggtga tggacagtcc gaactccctg ctgaggaccc cttcaacttc tacggagctt
361 ctcttctctc caaaggatcc ttctctaagg gccgcctcct catagacccg aactgtagtg
421 gccacagccc gcgcaccgcc cggcacgcac ctgcggtccg gaagttctcc cctgacctta
481 agttgcttaa ggatgtaaag attagcgtga gctttaccga gagctgcagg agtaaggaca
541 ggaaggtgct gtacacagga gcagagcgcg acgtgcgggc ggagtgcggt ctgctcctta
601 gccctgtcag tggggacgtg catgcttgtc cctttggcgg gagtgttggt gacggggtag
661 gcataggggg tgagagtgct gataagaagg atgaggagaa tgagctggat caggaaaaga
721 gagtggagta tgcagtgctc gatgagttag aagattttac tgacaatttg gagctagatg
781 aagaaggagc aggcgggttc acggctaaag caatcgttca gagagacaga gtggatgaag
841 aggccttgaa tttcccctac gaggatgact ttgacaacga tgtggatgct ctgctggaag
901 aaggcctttg tgcccccaaa aagaggcgaa cagaggaaaa atatggcgga gacagcgacc
961 atccgtccga tggagagaca agtgtgcagc cgatgatgac caagattaaa acagtgctca
1021 aaagtcgtgg ccgcccacct acagagccgc tgcccgacgg gtggatcatg acattccata
1081 actctggagt cccggtgtac ctacacagag agtctcgggt ggtcacctgg tccaggccat
1141 acttcttggg aacgggaagc atacggaaac acgaccctcc tctgagtagc atcccttgtc
1201 tgcattataa gaaaatgaag gacaacgagg aacgggagca aagcagtgac ctcaccccta
1261 gtggggatgt gtcccccgtc aagcccctga gccgatctgc agagctggag tttcccctgg
1321 atgagcctga ctctatgggt gctgacccgg ggcccccgga cgagaaagac ccactagggg
1381 ctgaggcagc ccctggggcc ctggggcagg tgaaggccaa agtcgaggtg tgcaaagatg
1441 aatccgttga tctcgaggaa tttcgaagct acctggagaa gcgttttgac tttgagcaag
1501 ttactgtgaa aaaattcagg acttgggctg agcggcggca attcaatcgg gaaatgaagc
1561 ggaagcaggc ggagtccgag aggcccatct tgccagccaa tcagaagctc attactttat
1621 cagtgcaaga tgcacccaca aagaaagagt ttgttattaa ccccaacggg aaatccgagg
1681 tctgcatcct gcacgagtac atgcagcgtg tcctcaaggt ccgccctgtc tataatttct
1741 ttgaatgtgc ccgagctaca ctggaaatcc tcatccctga ctttgttaaa cagacctctg
1801 aagagaagcc caaagacagt gaagaactcg agtattttaa ccacatcagc atcgaggact
1861 cgcgggtcta cgagctgacc agcaaggctg ggctgttgtc tccatatcag atcctccacg
1921 agtgccttaa aagaaaccat gggatgggtg acacgtctat caagtttgaa gtggttcctg
1981 ggaaaaacca gaagagtgaa tacgtcatgg cgtgtggcaa gcacacagtg cgcgggtggt
2041 gtaagaacaa gagagttgga aagcagttag cctcacagaa gatccttcag ctgctgcacc
2101 cacatgtcaa gaactggggg tctttactgc gcatgtatgg ccgtgagagc agcaagatgg
2161 tcaagcagga gacatcggac aagagtgtga ttgagctgca gcagtatgcc aagaagaaca
2221 agcccaacct gcacatcctc agcaagctcc aagaggagat gaagaggcta gctgaggaaa
2281 gggaggagac tcgaaagaag cccaagatgt ccattgtggc gtccgcccag cctggcggtg
2341 agcccctgtg caccgtggac gtgtgaggga ggtggcacgg gccagggcgc gggggccgcc
2401 agccgcactt ctgaggagac cagcagtcat gcatcgtgca ccacagtgtc aggcctccaa
2461 cccacgctcc ttccctgtgg ccaacctgtg ggcccggcct tagggtggag gctttagtgt
2521 acagggacag ccatggccac acagcacaca tgtggagcag cggctctccc tggaaagctc
2581 caggcctgaa tggatggact cagcgactgc accagtggca gctggtgact gtggacagtg
2641 gtggaccctg cttctgtgca cctgctgcag gctcttttta tgaaggcttt catgaatttt
2701 agtatgtaat acgcactgac gacacatgat gcttggatga cagatgagag gggatggctg
2761 agtcctgtgg ctggcccgtg atgccaggtg gcccatgtgc ccagggcgcc tgcagggctg
2821 ctacagggac ctggtcagga ggtgcacatg gtgccctgcc ctcacccacc ctctgtgttt
2881 ccccttcttt gaaaaggtag aagagaaagg aatattttaa acctttttgg cttaaacaga
2941 attttagcat cagaactagc tttctgggat tggaggcaaa ccatcaaggt ggtccctctc
3001 cagtctggac acgatgccag caaggatgac gtcctgccac ctcctggagt taccctggcc
3061 tcctagggtc cctttttctg atgaagtctt aattccctaa aagcgcctct ttggacactg
3121 aggccctctc tgcctttcct ggcctccggc aacagttttt tacaaagatt ttttgcagtc
3181 gagtccatat gtccacccat tgatttttaa agcttttgtg atattttagc attttgaaag
3241 actttcacag tgagagtaga aggtagattt ggaatcatgc attttagcaa gtggacttgt
3301 tgaaacagga agcaagggcc ttcagtgtag cccattcttg atccagagct gttgcctgtg
3361 acagcggttt ctctggatgt caaaggcagc tgcctggtgc ccagcttgct tctcgactgg
3421 tggcccctat gggtgggtgt gcgatggaaa tgtgttcctg ccggagtctg aggcaccagg
3481 gtgtgctcaa aggctggccc tggtggtgga ctggcacctg tgcagagtgc cgtgtgcttg
3541 tggtgcgcca tctgaagcaa gagtccagcg ttctgccgtg tctgtccccc accatgcccc
3601 ctacaggcgg tactgatggc gctttttttt ttttttctgt caggaaaaca atgttggcct
3661 gtgggccgcc cacaacatat ccttccctca ctacctgtgt gaccaaggtt ggcttctgtt
3721 gacctttaaa aaagaaaccc tcaactcaaa ttgctataat tagacacttg cttctgtctt
3781 gcctcctgtc tgcagctgtg aatagtcatt tgactgtgac tgttgccctt agccagccag
3841 atgcgcctgt gaaccaaagc ttcgtgcaca tgtgttcccc taaaggttgg ggagcctcgc
3901 tgtgtcttgc tgttcccagg caccaccaca gcaggtgctg ccatactctt gtggtctctg
3961 tgcgcccccc cccccccccc acccgtctgc caagcatggg tatgaatcgt gcacacagcc
4021 atgcttcaag gccggggcag gggagcctgt gctgatgcca tccagggcac tgggctgtgc
4081 ctggaaggcg agccttgatt gtctgaacac ataaagcaaa ctgtccagaa aaaaaaaaaa
4141 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//