LOCUS BC075858 4141 bp mRNA linear HUM 13-DEC-2004
DEFINITION Homo sapiens pleckstrin homology domain containing, family G (with
RhoGef domain) member 2, mRNA (cDNA clone IMAGE:3899827), partial
cds.
ACCESSION BC075858
VERSION BC075858.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4141)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4141)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (06-JUL-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 166 Row: b Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..4141
/db_xref="H-InvDB:HIT000265670"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3899827"
/tissue_type="Pancreas, epithelioid carcinoma"
/clone_lib="NIH_MGC_70"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..4141
/gene="PLEKHG2"
/gene_synonym="CLG"
/gene_synonym="FLJ00018"
/gene_synonym="FLJ22458"
/db_xref="GeneID:64857"
CDS <1..3764
/gene="PLEKHG2"
/gene_synonym="CLG"
/gene_synonym="FLJ00018"
/gene_synonym="FLJ22458"
/codon_start=3
/product="PLEKHG2 protein"
/protein_id="AAH75858.1"
/db_xref="GeneID:64857"
/translation="RVAREIVETERAYVRDLRSIVEDYLGPLLDGGVLGLSVEQVGTL
FANIEDIYEFSSELLEDLENSSSAGGIAECFVQRSEDFDIYTLYCMNYPSSLALLREL
SLSPPAALWLQERQAQLRHSLPLQSFLLKPVQRILKYHLLLQELGKHWAEGPGTGGRE
MVEEAIVSMTAVAWYINDMKRKQEHAARLQEVQRRLGGWTGPELSAFGELVLEGAFRG
GGGGGPRLRGGERLLFLFSRMLLVAKRRGLEYTYKGHIFCCNLSVSESPRDPLGFKVS
DLTIPKHRHLLQAKNQEEKRLWIHCLQRLFFENHPASIPAKAKQVLLENSLHCAPKSK
PVLEPLTPPLGSPRPRDARSFTPGRRNTAKPGFKHAGSEGELYPPESQPPVSGSAPPE
DLEDAGPPTLDPSGTSITEEILELLNQRGLRDPGPSTHDIPKFPGDSQVPGDSETLTF
QALPSRDSSEEEEEEEEGLEMDERGPSPLHVLEGLESSIAAEMPSIPCLTKIPDVPNL
PEIPSHCEIPEGSRLPSLSDISDVFEMPCLPAIPSVPNTPSLSSTPTLSCDSWLQGPL
QEPAEAPATRRELFSGSNPGKLGEPPSGGKAGPEEDEEGVSFTDFQPQDVTQHQGFPD
ELAFRSCSEIRSAWQALEQGQLARPGFPEPLLILEDSDLGGDSGSGKAGAPSSERTAS
RVRELARLYSERIQQMQRAETRASANAPRRRPRVLAQPQPSPCLPQEQAEPGLLPAFG
HVLVCELAFPLTCAQESVPLGPAVWVQAAIPLSKQGGSPDGQGLHVSNLPKQDLPGIH
VSAATLLPEQGGSRHVQAPAATPLPKQEGPLHLQVPALTTFSDQGHPEIQVPATTPLP
EHKSHMVIPAPSTAFCPEQGHCADIHVPTTPALPKEICSDFTVSVTTPVPKQEGHLDS
ESPTNIPLTKQGGSRDVQGPDPVCSQPIQPLSWHGSSLDPQGPGDTLPPLPCHLPDLQ
IPGTSPLPAHGSHLDHRIPANAPLSLSQELPDTQVPATTPLPLPQVLTDIWVQALPTS
PKQGSLPDIQGPAAAPPLPEPSLTDTQVQKLTPSLEQKSLIDAHVPAATPLPERGGSL
DIQGLSPTPVQTTMVLSKPGGSLASHVARLESSDLTPPHSPPPSSRQLLGPNAAALSR
YLAASYISQSLARRQGPGGGAPAASRGSWSSAPTSRASSPPPQPQPPPPAARRLSYAT
TVNIHVGGGGRLRPAKAQVRLNHPALLASTQESMGLHRAQGAPDAPFHM"
BASE COUNT 898 a 1326 c 1118 g 799 t
ORIGIN
1 agcgtgtggc ccgggagatc gtggagacag aacgggccta tgtcagggac ctccgcagca
61 tcgtggagga ctacctgggc cctctgctgg acggcggggt cctggggctg agcgtggagc
121 aggtgggcac gctgtttgcc aacattgagg acatctacga gttcagcagc gagctcctgg
181 aggacttgga gaacagcagc agcgccgggg gtattgccga gtgcttcgtg cagaggagcg
241 aggattttga catctacaca ttgtactgca tgaactaccc gagctccctg gccctgctcc
301 gggagctgtc gttgtctccg ccagcagccc tgtggctgca ggagcgccag gcccagcttc
361 gccactcgct gcccctgcag agcttcctgc tgaaacctgt ccagcgcatt ctcaagtacc
421 atctgctgct gcaggaacta gggaagcact gggcggaggg cccaggcact gggggtcgcg
481 agatggtgga ggaagctatt gtgtccatga cagcggttgc ctggtacatc aacgacatga
541 agcgcaagca ggagcatgca gcgcgcctcc aggaagtgca gcggcggctg ggtggctgga
601 ccggaccaga gctcagtgct tttggggaac tggtgttgga gggcgcgttc cgaggaggcg
661 gagggggtgg cccccggcta cgagggggtg agcggctgct cttcctgttc tctcggatgc
721 tgctggtggc caagcgcagg gggctggagt acacctacaa aggccacatc ttctgctgca
781 acctgagcgt gagcgagagt ccccgagacc ctctagggtt caaggtgtct gatctgacca
841 ttcccaagca cagacacctg ctccaggcca agaaccaaga agagaagagg ctgtggattc
901 actgtctcca gcgcctcttc tttgagaacc accctgcctc catccctgcc aaggcaaagc
961 aagttctcct tgaaaacagc ctgcattgtg cccccaaaag taagcctgtc ctagagcccc
1021 tgacaccccc acttgggtct cctcgacctc gagatgctag aagttttacc cctgggcgaa
1081 ggaacacagc taagcctgga ttcaagcacg ctggcagcga aggggaactc taccctccag
1141 aatctcagcc accagtttca ggctctgcac cccctgagga cctggaggat gctggacccc
1201 caacactgga cccctctggg acctcaatca ctgaagaaat cctggaactg ctgaatcagc
1261 gaggccttcg agatccaggg ccgtccaccc atgacattcc caagttcccc ggagactccc
1321 aggtgcctgg cgacagcgaa accctcacat tccaagccct gcccagccgg gactcttcag
1381 aagaggagga ggaggaagag gaagggctgg agatggatga acgggggcct tccccactcc
1441 acgtcctgga agggctcgaa agttccattg cagctgaaat gcccagcatt ccctgcctta
1501 ccaaaattcc tgacgtgccc aaccttcctg aaattcccag ccactgtgaa attcccgaag
1561 gttctcgcct tcctagtctc tctgacattt ccgatgtttt tgagatgccc tgccttccag
1621 ccatacctag tgtccccaac acccccagtc tgtctagcac tcccaccctc tcctgtgact
1681 cctggctcca agggcctctg caggaaccag ctgaggctcc agccaccagg agagaactgt
1741 tttctgggag caatcctggg aaactgggag agccgccttc aggaggcaag gcagggccag
1801 aggaggatga agaaggggta tcattcacag acttccagcc ccaggatgtc acccaacatc
1861 agggattccc agatgagctg gcattccgct cttgctcaga aatccggagc gcctggcagg
1921 cattggaaca gggacagctg gcccggccag gcttcccaga gccactgctg atcctggagg
1981 attcggatct gggtggagac agcgggagcg ggaaggcagg agccccgagt tcagaaagga
2041 cggcgtcccg agtgcgagag ctggcccggc tttacagcga gcggatccag cagatgcagc
2101 gggcggagac tcgggcatca gccaatgccc cgcgccgccg gcctcgggtt ctggcccaac
2161 cccagccatc cccctgtctg ccccaggagc aggcagagcc agggctcctg cctgcctttg
2221 gacacgtgct ggtatgtgag ctggccttcc cactgacatg tgcccaggag tctgtccccc
2281 tgggtcctgc tgtctgggtt caagctgcca tacctttgtc aaagcaggga ggcagcccgg
2341 atggccaggg tctacatgtt tccaatttgc ctaagcaaga ccttccgggc atccacgttt
2401 cagctgctac ccttttgcct gagcaaggag gttcccggca tgtccaggct ccagccgcca
2461 cacctttgcc caagcaagaa ggccccctgc acctccaggt gccggctctt acaactttct
2521 ctgatcaagg ccacccagaa atccaagttc cagccaccac tcctttgcct gagcataaaa
2581 gtcacatggt tataccagct ccatccaccg ccttttgtcc tgagcaggga cactgtgcgg
2641 acatccacgt tcccaccact ccagctttgc ccaaggagat ttgttctgat ttcacagttt
2701 cagtcaccac ccctgtgccc aagcaagaag gtcacctaga cagcgagagc ccaaccaata
2761 tcccactgac aaagcaagga ggttccaggg atgttcaggg cccagaccct gtctgcagtc
2821 aacccatcca gcctttgtct tggcatggaa gcagcctgga tccccagggc ccaggcgaca
2881 ccctaccacc cttgccatgt cacctcccag accttcagat tccaggtacc tcacctttgc
2941 ctgcacatgg aagccacctg gaccatcgga tcccagccaa cgccccactg tctttgtccc
3001 aggagctccc agacactcag gttccagcta ccacaccttt gcccctgcca caagtcctca
3061 cagacatctg ggtccaagcc ctcccaactt cacccaagca gggaagcctc ccagacatcc
3121 agggtccagc ggctgcacct ccacttccgg agccaagcct tacagataca caggtccaaa
3181 aactcacacc ttcgttggag cagaagagcc tcatagatgc ccatgttcca gctgccacac
3241 ctttacctga gagaggaggc tctctagaca ttcagggcct ctcacccacc ccagttcaga
3301 ccaccatggt tttgtccaaa ccaggaggct ccttagcctc tcacgttgcc aggttggagt
3361 cttcagactt gacgccacct catagtcccc caccttccag ccgtcagctc ctgggcccca
3421 atgcagctgc cctctccaga tacctggcag cctcatatat cagccaaagc ctggctcggc
3481 ggcaggggcc tgggggaggg gcccccgcag cctcccgggg ctcctggtcc tctgctccca
3541 cgtcacgggc atcttcgccg cccccccagc cccagccacc acctcccgca gccaggcggc
3601 tcagctatgc cacgacggtt aacatccacg tgggcggggg tgggcggctg cggccagcca
3661 aggcccaggt ccggttgaac caccctgctc tcttggcctc cacacaggaa tctatgggcc
3721 ttcacagggc ccagggggct cctgatgccc ccttccacat gtgagccagg acatgaggct
3781 tccctgaagc aaggatttca gccagatgcc atagaccctc agaacttgac ctggaagtcc
3841 agacactgaa cgcaggcctc aaaactgctg cggccttcca actcctggta tctgcatcgg
3901 cgaatggccc ttcttgcctt gatccacagg gatggggaag ggaatgtcat taatgttttg
3961 ttaatactga ttctttcatg caatgatgtg tattttccca ttctggaggc tgtgggagat
4021 gacaagacaa tgaatgggaa ggtctgacac agaacaaatc agcggttctg aaagcttggg
4081 gaatctcaga ctcctttgag aattattgga aaatggaccc actataaaaa aaaaaaaaaa
4141 a
//